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Effect of Media, Temperature, Light, pH and Nutrient Source on Growth and Development of Bipolaris oryzae Causing Brown Leaf Spot of Paddy

2018; Excellent Publishers; Volume: 7; Issue: 07 Linguagem: Inglês

10.20546/ijcmas.2018.707.203

2319-7706

C. Channakeshava N.S. Pankaja,

Fungal Biology and Applications

The pathogen Bipolaris oryzae was subjected to different cultural conditions viz., media, temperature, pH, light and nutrient source under in vitro conditions. The maximum radial growth of 84.83 mm was recorded on paddy leaf extract agar followed by potato dextrose agar (61.33 mm). On this medium, the colony appeared greyish-white to dark brown, thick, leathery slightly raised and profuse mycelia with brown colored conidia. Similarly on liquid media, maximum dry mycelial weight of 113.06 mg was recorded on paddy leaf extract broth followed by potato dextrose broth (98.28 mg).Maximum radial growth of 70.67 mm,62.83 mmand68.00 mm was recorded at 30°C,25°C and complete dark regime, respectively. Maximum dry mycelial weight of 113.0 mg was recorded at pH 7.0 followed by 103.0 mg at pH 7.5 and 97.32 mg at pH 6.5.Among the different carbon and nitrogen sources maximum growth was recorded in glucose (89.57, 102.86, 112.73 and 128.88 mg)and ammonium peptone (79.10, 97.43, 103.61 and 115.24 mg) at 0.5, 1.0, 1.5 and 2.0% concentration respectively.