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Temporal changes of major protein concentrations in preterm and term human milk. A prospective cohort study

2018; Elsevier BV; Volume: 38; Issue: 4 Linguagem: Inglês

10.1016/j.clnu.2018.07.016

1532-1983

Clara L. García‐Ródenas, Carlos Antonio De Castro, Rosemarie Jenni, Sagar K. Thakkar, Lydie Beauport, Jean‐François Tolsa, Céline Fischer-Fumeaux, Michael Affolter,

Neonatal Respiratory Health Research

Background Proteins are major contributors to the beneficial effects of human milk (HM) on preterm infant health and development. Alpha-lactalbumin, lactoferrin, serum albumin and caseins represent approximately 85% of the total HM protein. The temporal changes of these proteins in preterm (PT) HM and its comparison with term (T) HM is poorly characterized. Aims To quantify and compare the temporal changes of the major proteins in PT HM and T HM. Methods HM was collected for 4 months postpartum at 12 time points for PT HM (gestational age 28 0/7–32 6/7 weeks; 280 samples) and for 2 months postpartum at 8 time points for T HM (gestational age 37 0/7–41 6/7 weeks; 220 samples). Proteins were measured with a micro-fluidic LabChip system. Results Casein, alpha-lactalbumin and lactoferrin decreased with advancing stages of lactation in PT and T HM, whereas serum albumin remained stable. Only marginal differences between PT and T HM were observed for alpha-lactalbumin during postpartum weeks 3–5 and for serum albumin at the first week. However, a comparison of HM provided to preterm and term infants at the same postmenstrual ages revealed that alpha-lactalbumin contents were significantly lower in PT HM than in T HM during the 39–48 postmenstrual weeks. Conclusions This study provides comprehensive information of the longitudinal changes of major proteins in PT and T HM, and suggests limited availability of alpha-lactalbumin, a nutritionally important protein, in breastfed PT infants after reaching the term corrected age. This information may be important to optimize HM protein fortification, although its biological relevance needs to be confirmed by intervention studies. Clinical trial registry ClinicalTrials.gov (NCT02052245), https://clinicaltrials.gov/ct2/show/NCT02052245.