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In vitro evaluation of barrier function against oral bacteria of dense and expanded polytetrafluoroethylene (PTFE) membranes for guided bone regeneration

2018; Wiley; Volume: 20; Issue: 5 Linguagem: Inglês

10.1111/cid.12629

1708-8208

Margarita Trobos, Annika Juhlin, Furqan A. Shah, Maria Hoffman, Herman Sahlin, Christer Dahlin,

Dental materials and restorations

Abstract Aim This study evaluates biofilm formation and barrier function against Streptococcus oralis of nonresorbable polytetrafluoroethylene (PTFE) guided bone regeneration membranes having expanded (e‐PTFE) and dense (d‐PTFE) microstructure. Materials and Methods Three e‐PTFE membranes of varying openness, one d‐PTFE membrane, and commercially pure titanium discs were evaluated. All e‐PTFE membranes consisted of PTFE nodes interconnected by fibrils. The d‐PTFE membrane was fibril‐free, with large evenly spaced indentations. The surfaces were challenged with S. oralis and incubated statically for 2‐48h. Bacterial colonization, viability, and penetration were evaluated. Results S. oralis numbers increased over time on all surfaces, as observed using scanning electron microscopy, while cell viability decreased, as measured by colony forming unit (CFU) counting. At 24h and 48h, biofilms on d‐PTFE were more mature and thicker (tower formations) than on e‐PTFE, where fewer layers of cells were distributed mainly horizontally. Biofilms accumulated preferentially within d‐PTFE membrane indentations. At 48h, greater biofilm biomass and number of viable S. oralis were found on d‐PTFE compared to e‐PTFE membranes. All membranes were impermeable to S. oralis cells. Conclusions All PTFE membranes were effective barriers against bacterial passage in vitro . However, d‐PTFE favored S. oralis biofilm formation.