Portal de Periódicos da CAPES

Neocosmospora rubicola Causing Stem Rot of Pitaya ( Hylocereus costaricensis ) in China

2018; American Phytopathological Society; Volume: 102; Issue: 12 Linguagem: Inglês

10.1094/pdis-09-17-1469-pdn

1943-7692

Fangliang Zheng, Guohui Xu, Fangliang Zheng, X. F. Ding, C. P. Xie,

Date Palm Research Studies

HomePlant DiseaseVol. 102, No. 12Neocosmospora rubicola Causing Stem Rot of Pitaya (Hylocereus costaricensis) in China PreviousNext DISEASE NOTES OPENOpen Access licenseNeocosmospora rubicola Causing Stem Rot of Pitaya (Hylocereus costaricensis) in ChinaF. Zheng, G. Xu, F. Q. Zheng, X. F. Ding, and C. P. XieF. Zhenghttp://orcid.org/0000-0002-3299-4543, G. Xuhttp://orcid.org/0000-0002-8642-8221, F. Q. Zheng, X. F. Ding, and C. P. Xie†Corresponding author: C. P. Xie; E-mail: E-mail Address: xiechangping009@163.comAffiliationsAuthors and Affiliations F. Zheng G. Xu F. Q. Zheng X. F. Ding C. P. Xie † , Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education/Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. Published Online:10 Oct 2018https://doi.org/10.1094/PDIS-09-17-1469-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Pitaya (Hylocereus costaricensis) is a tropical and subtropical plant of the family Cactaceae. In June 2016, a stem rot disease was observed affecting over 15% of a pitaya planting in Dongfang (Hainan Province). Symptomatic plants initially showed yellowing of stem base, and lesions developed that were shriveled, curling, and soft. The vascular bundles of stems changed internally from pale yellow to reddish brown. As the disease developed, it sometimes developed into root rot. Pitaya stems with typical symptoms were washed with tap water. Small pieces of tissue (4 × 4 mm) were cut from the margin of a stem lesion and were disinfected in alcohol (75%) for 30 s and HgCl2 (0.1%) for 1 min, followed by washing three times in sterile water. The disinfected tissues were placed on potato dextrose agar (PDA) and incubated at 28°C. Twenty-one isolates were obtained, and all had similar cultural and morphological characteristics; two of these (HLG0112 and HLG0117) were selected for further characterization. Seven days after incubation, colonies were fast growing with white aerial mycelia and a canary yellow color on the reverse side. Two types of spores were produced on PDA: microconidia were oval to kidney shaped, single celled, 7.0 to 13.5 × 2.2 to 3.4 μm. Macroconidia (4.9 to 6.3 × 31.5 to 45.7 µm) had two to five septa, sickle shaped, slightly curved. Based on morphology, the fungus was identified as Fusarium-like. Four gene regions of isolate HLG0112 were sequenced, including the ribosomal internal transcribed spacer (ITS), calmodulin (cmdA), 28S large subunit nrDNA (LSU), and histone H3 (his3) regions. The gene regions were amplified and sequenced using the primers ITS1/ITS4, CYL-228F/CYL2Rd, LR0R/LR7, and CYLH3F/CYLH3R (Lombard et al. 2015). The ITS, LSU, cmdA, and his3 sequences (MH277488, MH277489, MH332387, and MH297490) all had 99% identity to Neocosmospora rubicola (CBS 320.73; GenBank accession nos. KM231799, KM231666, KM231371, and KM231523, respectively). Based on morphological and molecular characteristics, the fungus was identified as N. rubicola L. Lombard & Crous (Lombard et al. 2015). Pathogenicity tests were conducted by placing 5-mm-diameter mycelial plugs obtained from 7-day-old cultures on the stem base. Controls were treated with PDA plugs. Ten plants were placed into each group, which were maintained in a growth chamber at 28°C and covered with a plastic bag to maintain a high relative humidity. After 5 to 10 days, symptoms identical to those of field samples developed on all the inoculated plants, whereas the control plants remained asymptomatic. The pathogenicity tests were conducted three times. The same fungus was reisolated from the infected plants, and the identity was confirmed by cultural and morphological characters, confirming Koch's postulates. Several Neocosmospora species have recently been reported from diseases of other tropical and subtropical plants (Sandoval-Denis et al. 2018). Fusarium fujikuroi has been reported to cause stem rot lesion of H. polyrhizus in Malaysia (Masratul Hawa et al. 2017) and Sclerotium rolfsii in China (Zheng et al. 2018). To our knowledge, this is the first report of N. rubicola causing stem rot of pitaya in China.References:Lombard, L., et al. 2015. Stud. Mycol. 80:189. https://doi.org/10.1016/j.simyco.2014.12.002 Crossref, ISI, Google ScholarMasratul Hawa, M., et al. 2017. Ann. Appl. Biol. 170:434. https://doi.org/10.1111/aab.12348 Crossref, ISI, Google ScholarSandoval-Denis, N., et al. 2018. Persoonia 40:1. Crossref, ISI, Google ScholarZheng, F., et al. 2018. Plant Dis. 102:441. https://doi.org/10.1094/PDIS-06-17-0869-PDN Link, ISI, Google ScholarFunding: Funding was provided by Hainan Province Natural Science Foundation of China (grant no. 20163040).DetailsFiguresLiterature CitedRelated Vol. 102, No. 12 December 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Nov 2018Published: 10 Oct 2018First Look: 3 Aug 2018Accepted: 4 Jul 2018 Pages: 2653-2653 Information© 2018 The American Phytopathological SocietyFundingHainan Province Natural Science Foundation of ChinaGrant/Award Number: 20163040Cited byFungal communities in Brazilian cassava tubers and food productsInternational Journal of Food Microbiology, Vol. 384Neocosmospora rubicola, a stem rot disease in potato: Characterization, distribution and management11 August 2022 | Frontiers in Microbiology, Vol. 13Discovering the Diversity of Arbuscular Mycorrhizal Fungi Associated with Two Cultivation Practices of Theobroma cacao12 August 2022 | Diversity, Vol. 14, No. 8Fungal and Oomycete Diseases of Minor Tropical Fruit Crops11 April 2022 | Horticulturae, Vol. 8, No. 4A Bacterial Consortium and Synthetic Fertilizer Based Biocontrol Approach Against Potato Rot Disease "Neocosmospora rubicola"8 March 2022 | Frontiers in Agronomy, Vol. 4Vertically transmitted microbiome protects eggs from fungal infection and egg failure16 June 2021 | Animal Microbiome, Vol. 3, No. 1Effect of Calcium Cyanamide on Soil Fungal Community in Successive Tea-Cuttings Nursery29 July 2021 | Agriculture, Vol. 11, No. 8First Inventory of Fungi in Symptomless and Symptomatic Chinese Mesona Indicates Phytopathological ThreatChung-Wei Hsieh, Ying-Ying Chuang, Ming-Zhe Lee, and Roland Kirschner13 July 2020 | Plant Disease, Vol. 104, No. 9Rehmannia glutinosa Replant Issues: Root Exudate-Rhizobiome Interactions Clearly Influence Replant Success30 June 2020 | Frontiers in Microbiology, Vol. 11Diseases of dragon fruit (Hylocereus species): Etiology and current management optionsCrop Protection, Vol. 126