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Quillworts from the Amazon: A multidisciplinary populational study on Isoetes serracarajensis and Isoetes cangae

2018; Public Library of Science; Volume: 13; Issue: 8 Linguagem: Inglês

10.1371/journal.pone.0201417

1932-6203

Gisele Nunes, Renato Oliveira, José Tasso Felix Guimarães, Ana María Giulietti, Cecílio Fróis Caldeira, Santelmo Vasconcelos, Eder S. Pires, Mariana Costa Dias, Maurício Takashi Coutinho Watanabe, Jovani B. S. Pereira, Rodolfo Jaffé, Cinthia Helena M. M. Bandeira, Nelson Carvalho-Filho, Edilson Freitas da Silva, Tarcísio Magevski Rodrigues, Fernando Marino Gomes dos Santos, Taís Nogueira Fernandes, Alexandre Castilho, Pedro Walfir Martins e Souza Filho, Vera Lùcia Imperatriz-Fonseca, José Oswaldo Siqueira, Ronnie Alves, Guilherme Oliveira,

Plant Parasitism and Resistance

Isoetes are ancient quillworts members of the only genus of the order Isoetales. The genus is slow evolving but is resilient, and widespread worldwide. Two recently described species occur in the Eastern Brazilian Amazon, Isoetes serracarajensis and Isoetes cangae. They are found in the ironstone grasslands known as Canga. While I. serracarajensis is present mostly in seasonal water bodies, I. cangae is known to occur in a single permanent lake at the South mountain range. In this work, we undertake an extensive morphological, physiological and genetic characterization of both species to establish species boundaries and better understand the morphological and genetic features of these two species. Our results indicate that the morphological differentiation of the species is subtle and requires a quantitative assessment of morphological elements of the megaspore for diagnosis. We did not detect differences in microspore output, but morphological peculiarities may establish a reproductive barrier. Additionally, genetic analysis using DNA barcodes and whole chloroplast genomes indicate that although the plants are genetically very similar both approaches provide diagnostic characters. There was no indication of population structuring I. serracarajensis. These results set the basis for a deeper understanding of the evolution of the Isoetes genus.