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First Report of the Occurrence of Bacterial Leaf Streak of Corn Caused by Xanthomonas vasicola pv. vasculorum in Brazil
2018; American Phytopathological Society; Volume: 103; Issue: 1 Linguagem: Inglês
10.1094/pdis-06-18-1100-pdn
ISSN1943-7692
AutoresRui Pereira Leite, Adriano Augusto de Paiva Custódio, T. Madalosso, Renata Rodrigues Robaina, Izabela Moura Duin, Vanessa Hitomi Sugahara,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 103, No. 1First Report of the Occurrence of Bacterial Leaf Streak of Corn Caused by Xanthomonas vasicola pv. vasculorum in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of the Occurrence of Bacterial Leaf Streak of Corn Caused by Xanthomonas vasicola pv. vasculorum in BrazilR. P. Leite Jr., A. A. P. Custódio, T. Madalosso, R. R. Robaina, I. M. Duin, and V. H. SugaharaR. P. Leite Jr.†Corresponding author: R. P. Leite Jr.; E-mail: E-mail Address: [email protected]http://orcid.org/0000-0001-8211-7840, A. A. P. Custódio, T. Madalosso, R. R. Robaina, I. M. Duin, and V. H. SugaharaAffiliationsAuthors and Affiliations R. P. Leite Jr. † A. A. P. Custódio , Instituto Agronômico do Paraná (IAPAR), Londrina, PR, Brazil T. Madalosso , Copacol Cooperativa Agroindustrial Consolata, Cafelândia, PR, Brazil R. R. Robaina I. M. Duin , IAPAR, Londrina, PR, Brazil, and Fellow of Fundação Araucária, Curitiba, PR, Brazil V. H. Sugahara , IAPAR, Londrina, PR, Brazil. Published Online:25 Oct 2018https://doi.org/10.1094/PDIS-06-18-1100-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Bacterial leaf streak of corn, caused by Xanthomonas vasicola pv. vasculorum, was recently reported in the United States and Argentina (Korus et al. 2017; Lang et al. 2017; Plazas et al. 2018). Further, the disease has reached epidemic levels in some corn-growing states of the United States (Korus et al. 2017; Lang et al. 2017). In April 2018, diseased corn leaf samples were collected in commercial fields in the western region of the state of Paraná, Brazil. The leaves with initial symptoms of the disease had translucent yellow to brown narrow linear lesions with irregular margins, confined to the interveinal spaces. Older symptoms on highly susceptible corn hybrids had coalescing long lesions of dark coloration, reaching large extension along the leaf blade. Preliminary examinations did not show any structure of fungi but revealed the presence of bacterial ooze. Bacterial isolates established on nutrient agar medium produced yellow mucoid colonies typical of bacteria of the genus Xanthomonas. The pathogenicity of the bacterial isolates was confirmed by spray inoculation of a 108 CFU/ml suspension in plants at the V4 stage of the Zea mays hybrid JM 2M77, kept under greenhouse conditions. Lesions similar to those observed in the samples from commercial corn fields started to show up 9 days after inoculation. We reisolated consistently from the affected leaf tissues bacteria identical to those originally inoculated. The bacterium obtained had a rod-shaped cell, was gram negative, nonfluorescent and nonfermentative. Based on the analysis of polymerase chain reaction amplified sequences of the 16S rDNA region, using the universal primers fD1 and rD1 (Weisburg et al. 1991), the bacterial isolates showed genetic similarity of 99% with X. vasicola pv. vasculorum strain SAM119 (NCCPB no. 4614), isolated from corn in South Africa, the pathotype strain of the pathovar (Lang et al. 2017). Further, a neighbor-joining phylogenetic tree reconstructed based on partial 16S rDNA sequences alignment using MEGA7 (Kumar et al. 2016) confirmed that our bacterial strains clustered with X. vasicola pv. vasculorum strain SAM119. The sequences of two bacterial strains established in our studies were deposited in GenBank as accessions MH497007 and MH497008. The disease was present in commercial fields in at least eight municipalities of corn-producing regions of the state of Paraná, Brazil. In addition, bacterial leaf streak of corn was detected under natural conditions on Z. mays plants of at least 30 different commercial hybrids. Although the occurrence of X. vasicola pv. vasculorum causing bacterial leaf streak of corn has been suspected in Brazil for previous years, this is the first confirmed report.References:Korus, K., et al. 2017. Plant Dis. 101:1030. https://doi.org/10.1094/PDIS-10-16-1426-PDN Link, ISI, Google ScholarKumar, S., et al. 2016. Mol. Biol. Evol. 33:1870. https://doi.org/10.1093/molbev/msw054 Crossref, ISI, Google ScholarLang, J. M., et al. 2017. Phytopathol. 107:1312. https://doi.org/10.1094/PHYTO-05-17-0168-R Link, ISI, Google ScholarPlazas, M. C., et al. 2018. Plant Dis. 102:1026. https://doi.org/10.1094/PDIS-10-17-1578-PDN Link, ISI, Google ScholarWeisburg, W. G., et al. 1991. J. Bacteriol. 173:697. https://doi.org/10.1128/jb.173.2.697-703.1991 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 103, No. 1 January 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 4 Jan 2019Published: 25 Oct 2018First Look: 4 Aug 2018Accepted: 3 Aug 2018 Pages: 145-145 Information© 2019 The American Phytopathological SocietyCited byIn vitro sensitivity of Xanthomonas vasicola pv. vasculorum and the control of bacterial leaf streak of corn with copper oxychloride alone and in mixtures with mancozeb and fluxapyroxad20 August 2022 | European Journal of Plant Pathology, Vol. 164, No. 2Occurrence and detection of Xanthomonas vasicola pv. vasculorum in naturally contaminated maize seeds in Brazil6 June 2022 | Tropical Plant Pathology, Vol. 47, No. 5Xanthomonas vasicola pv. holcicola (streaky spot of sorghum and Sudan grass)CABI Compendium, Vol. CABI CompendiumXanthomonas axonopodis pv. vasculorum (sugarcane gumming disease)CABI Compendium, Vol. CABI CompendiumXanthomonas vasicola pv. vasculorum (bacterial leaf streak of corn)CABI Compendium, Vol. CABI CompendiumSensibility of Xanthomonas vasicola pv. vasculorum to chemicals and efficiency of the chemical control of bacterial leaf streak on corn plants24 May 2022 | Acta Scientiarum. 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Jackson-Ziems5 May 2020 | Phytopathology®, Vol. 110, No. 6Genomic Acquisitions in Emerging Populations of Xanthomonas vasicola pv. vasculorum Infecting Corn in the United States and ArgentinaAlvaro L. Perez-Quintero, Mary Ortiz-Castro, Jillian M. Lang, Adrien Rieux, Guangxi Wu, Sanzhen Liu, Toni A. Chapman, Christine Chang, Janet Ziegle, Zhao Peng, Frank F. White, Maria Cristina Plazas, Jan E. Leach, and Kirk Broders24 March 2020 | Phytopathology®, Vol. 110, No. 6Alternative Hosts in the Families Poaceae and Cyperaceae for Xanthomonas vasicola pv. vasculorum, Causal Agent of Bacterial Leaf Streak of CornT. Hartman, B. Tharnish, J. Harbour, G. Y. Yuen, and T. A. Jackson-Ziems17 March 2020 | Phytopathology®, Vol. 110, No. 6Occurrence in Seeds and Potential Seed Transmission of Xanthomonas vasicola pv. vasculorum in Maize in the United StatesSilvina L. Arias, Charles C. Block, Derrick A. Mayfield, Gem Santillana, Michael J. Stulberg, Kirk D. Broders, Tamra A. Jackson-Ziems, and Gary P. Munkvold5 May 2020 | Phytopathology®, Vol. 110, No. 6Genomics-Informed Molecular Detection of Xanthomonas vasicola pv. vasculorum Strains Causing Severe Bacterial Leaf Streak of CornMichael J. Stulberg, Gem Santillana, David J. Studholme, Beth Kasiborski, Mary Ortiz-Castro, Kirk Broders, Silvina Arias, Charles Block, Gary Munkvold, and John Rascoe10 January 2020 | Phytopathology®, Vol. 110, No. 6Development and validation of a diagrammatic scale for the assessment of the severity of bacterial leaf streak of corn11 May 2020 | European Journal of Plant Pathology, Vol. 157, No. 2Identification of quantitative trait loci associated with maize resistance to bacterial leaf streak9 March 2020 | Crop Science, Vol. 60, No. 1
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