Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Transcriptome analysis of Corynebacterium pseudotuberculosis biovar Equi in two conditions of the environmental stress

2018; Elsevier BV; Volume: 677; Linguagem: Inglês

10.1016/j.gene.2018.08.028

1879-0038

Anne Cybelle Pinto Gomide, Izabela Coimbra Ibraim, Jorianne Thyeska Castro Alves, Pablo Gomes de Sá, Yuri Rafael de Oliveira Silva, Mariana Passos Santana, Wanderson Marques da Silva, Edson Luiz Folador, Diego Mariano, Thiago Luiz de Paula Castro, Silvanira Barbosa, Fernanda Alves Dorella, Alex F. Carvalho, Felipe Luiz Pereira, Carlos Augusto Gomes Leal, Henrique César Pereira Figueiredo, Vasco Azevedo, Artur Silva, Adriana Ribeiro Carneiro Folador,

Veterinary medicine and infectious diseases

Corynebacterium pseudotuberculosis has been widely studied in an effort to understand its biological evolution. Transcriptomics has revealed possible candidates for virulence and pathogenicity factors of strain 1002 (biovar Ovis). Because C. pseudotuberculosis is classified into two biovars, Ovis and Equi, it was interesting to assess the transcriptional profile of biovar Equi strain 258, the causative agent of ulcerative lymphangitis. The genome of this strain was re-sequenced; the reassembly was completed using optical mapping technology, and the sequence was subsequently re-annotated. Two growth conditions that occur during the host infection process were simulated for the transcriptome: the osmotic and acid medium. Genes that may be associated with the microorganism's resilience under unfavorable conditions were identified through RNAseq, including genes present in pathogenicity islands. The RT-qPCR was performed to confirm the results in biological triplicate for each condition for some genes. The results extend our knowledge of the factors associated with the spread and persistence of C. pseudotuberculosis during the infection process and suggest possible avenues for studies related to the development of vaccines, diagnosis, and therapies that might help minimize damage to agribusinesses.