Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Innovative phytoformulation containing capsaicinoids: Microparticles development, analytical method validation, and anti-ulcer effect

2018; SAGE Publishing; Volume: 14; Issue: 56 Linguagem: Inglês

10.4103/pm.pm_160_17

0976-4062

PauloV Farago, MartinhaA Almeida, JessicaM Nadal, Traudi Klein, JosianeP de Paula, JaneM Budel, Andressa Novatski, ElianeA Campesatto,

Herbal Medicine Research Studies

Pharmacognosy Magazine,2018,14,56,290-296.DOI:10.4103/pm.pm_160_17Published:August 2018Type:Original ArticleAuthors:Martinha A Almeida, Jessica M Nadal, Traudi Klein, Josiane P de Paula, Jane M Budel, Andressa Novatski, Eliane A Campesatto, and Paulo V Farago Author(s) affiliations:Martinha A Almeida1, Jessica M Nadal1, Traudi Klein1, Josiane P de Paula1, Jane M Budel1, Andressa Novatski2, Eliane A Campesatto3, Paulo V Farago1 1 Department of Pharmaceutical Science, State University of Ponta Grossa, Ponta Grossa, Paraná 2 Department of Physics, State University of Ponta Grossa, Ponta Grossa, Paraná 3 Department of Pharmacy, Federal University of Alagoas, Maceió, Brazil, South American Abstract:Background: Capsicum annuum L. and Capsicum frutescens L., of family Solanaceae, contain capsaicinoids with several pharmacological effects. However, their pungency limits the long-term use through the gastrointestinal tract. Objective: To obtain phytoformulations of microparticles containing capsaicinoids for reducing their pungency by oral route to achieve an anti-ulcer effect. Materials and Methods: Microparticles of poly(ε-caprolactone) containing capsaicinoids were prepared by simple emulsion/solvent evaporation. An optimized reverse-phase high-performance liquid chromatography method with ultraviolet (UV) detection for quantifying capsaicinoids into microparticle phytoformulations was then developed and validated. Chromatographic conditions consisted of a C18 reverse-phase analytical column (250 mm × 4.60 mm, 5 μm) using a mixture of acetonitrile and water (70:30 v/v) adjusted to pH 4.5 as mobile phase at a flow rate of 0.75 mL/min with UV detection at 280 nm. The developed method was validated as per the ICH guidelines with respect to specificity, linearity, limit of quantification, limit of detection, accuracy, precision, and robustness. The gastroprotective activity of pure capsaicinoids and loaded microparticles against ethanol-induced gastric ulcer in rats was performed. Results: Microparticles were successfully obtained. Analytical validation provided suitable results regarding all parameters investigated. These phytoformulations presented suitable encapsulation efficiency higher than 90%. Regarding the ulcerative lesion index (ULI) scores, poly(ε-caprolactone) (PCL) microparticles containing 5% of capsaicinoids (ULI = 16.3 ± 1.8 points) was statistically similar (P > 0.05) to ranitidine (ULI = 15.3 ± 1.4 points) and omeprazole (ULI = 8.0 ± 1.2 points). Conclusion: Capsaicinoids-loaded PCL microparticles reveal the potential to be suitable candidates as controlled drug delivery system for the therapeutic management of ulcer. Keywords:Anti-ulcer activity, capsaicin, Dihydrocapsaicin, Gastric protection, Low-irritative oral preparation, Poly(ε-caprolactone)View:PDF (1.33 MB)