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Long noncoding RNA HOXB 13‐ AS 1 regulates HOXB 13 gene methylation by interacting with EZH 2 in glioma

2018; Wiley; Volume: 7; Issue: 9 Linguagem: Inglês

10.1002/cam4.1718

2045-7634

Yu Xiong, Kuang Wei, Shi-Gang Lu, Hua Guo, Miaojing Wu, Minhua Ye, Lei Wu,

RNA Research and Splicing

Abstract Dysregulation of long noncoding RNA s (lnc RNA s) has been implicated in human diseases, in particular, cancers. In this study, we determined the expression of an lnc RNA , HOXB 13‐ AS 1, involving in glioma. We showed that HOXB 13‐ AS 1 was significantly upregulated in glioma tissues and cells and was negatively correlated with its surrounding gene HOXB 13 levels. Functional experiments in vitro and in vivo revealed that high level of HOXB 13‐ AS 1 increased cell proliferation and tumor growth by promoting cell cycle progression. Conversely, knockdown of HOXB 13‐ AS 1 resulted in decreased cell proliferation and tumor growth. Mechanistically, we showed that HOXB 13‐ AS 1 overexpression increased DNMT 3B‐mediated methylation of adjacent gene HOXB 13 promoter by binding with the enhancer of zeste homolog 2 ( EZH 2) using bisulfite sequencing PCR ( BSP ), epigenetically suppressing HOXB 13 expression. Additionally, the interaction between HOXB 13‐ AS 1 and HOXB 13 was validated by RNA immunoprecipitation ( RIP ) and chromatin immunoprecipitation (Ch IP ) assays using antibody against to EZH 2. Taken together, our study indicated that HOXB 13‐ AS 1 could regulate HOXB 13 gene expression by methylation HOXB 13 promoter and acts as an epigenetic oncogenic in glioma.