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Evaluation of nanogold particles-based enzyme-linked immunosorbent assay for detection of hydatidosis

2018; Medknow; Volume: 35; Issue: 2 Linguagem: Inglês

10.4103/bmfj.bmfj_13_18

2357-0016

AmiraS ElGhannm, SamiaM Rashed, MonaE Naser, IbrahimR Bayoumi, NagwaS.M. Aly, WaleedE Mohamed,

Brucella: diagnosis, epidemiology, treatment

Background Use of nanotechnology in clinical diagnosis meets the demands for increased sensitivity and early detection in less time.Purpose The aim of this study was to evaluate the nanogold particles-based dot-enzyme-linked immunosorbent assay (ELISA) as a test for detection of protoscolices antigen in serum samples of infected animals in comparison with traditional dot-ELISA.Methods A total of 76 blood samples were collected and included in the study: 36 sample of hydatidosis confirmed cases, 20 samples infected with other parasitic infection except hydatidosis as positive controls, and 20 samples as negative controls. Dot-ELISA was applied using two polyclonal antibodies against protoscolices antigen, the purified immunoglobulin G (IgG) polyclonal antibodies, and peroxidase-conjugated IgG, whereas in the nanogold dot-ELISA, the purified IgG polyclonal antibodies were conjugated with nanogold particles.Results On detection of protoscolices antigen by dot-ELISA, 31 (86.1%) of 36 serum samples were found to be positive, whereas nanogold dot-ELISA gave 34 (94.4%) positive serum samples. Dot-ELISA with nanogold particles had higher values than dot-ELISA regarding sensitivity (94.4 vs. 86.1%), positive predictive value (94.4 vs. 93.9%), negative predictive value (78.3 vs. 90%), and accuracy (92.9 vs. 87.5%), but specificity (90%) was the same for both tests.Conclusion Nanoparticles-based dot-ELISA is superior over traditional dot-ELISA for the detection of protoscolices antigen in hydatidosis. Dot-ELISA is rapid and easy to perform and the results can be read with the naked eye, so it does not require expensive equipment.