A mitochondria-targeting ratiometric fluorescent probe for imaging hydrogen peroxide with long-wavelength emission and large Stokes shift
2018; Elsevier BV; Volume: 276; Linguagem: Inglês
10.1016/j.snb.2018.08.119
ISSN1873-3077
AutoresLong He, Xingjiang Liu, Yun Zhang, Lei Yang, Fang Qian, Yani Geng, Wenqiang Chen, Xiangzhi Song,
Tópico(s)Luminescence and Fluorescent Materials
ResumoHydrogen peroxide, one of the curial reactive oxygen species, is believed to involve in a wide range of physiological processes. In this work, we designed and synthesized a new fluorescent probe, HKB, for the detection of hydrogen peroxide, by taking advantage of the combination of ICT and ESIPT mechanisms. Different from other benzothiazole-based ratiometric probes, the intramolecular ESIPT process is preserved in probe HKB and the large Stokes shift is remained. Probe HKB displayed significant ratiometric fluorescence variations (I594nm/ I666nm) upon the addition of 50 equiv. of hydrogen peroxide under a single-wavelength excitation at 440 nm in PBS buffer (pH = 7.0, 10 mM, 30% CH3CN) and showed a large Stokes shift change from 102 nm to 254 nm. Moreover, this probe exhibited excellent mitochondria targeting property with a 0.94 Pearson’s colocalization coefficient using Rh123 as a reference. Gratifyingly, preliminary biological experiments indicated that probe HKB could sense both the exogenous and endogenous hydrogen peroxide in living HeLa cells.
Referência(s)