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Detection and Phylogenetic Characterization of Anaplasma capra: An Emerging Pathogen in Sheep and Goats in China

2018; Frontiers Media; Volume: 8; Linguagem: Inglês

10.3389/fcimb.2018.00283

2235-2988

Yongshuai Peng, Kunlun Wang, Shanshan Zhao, Yaqun Yan, Haiyan Wang, Jichun Jing, Fuchun Jian, Rongjun Wang, Longxian Zhang, Changshen Ning,

Toxin Mechanisms and Immunotoxins

Anaplasma capra is an emerging pathogen, which can infect ruminants and human. This study was conducted to determine the occurrence of Anaplasma capra in blood samples of sheep and goats in China. Using nested polymerase chain reaction (nested-PCR) targeting the gltA gene and conventional PCR targeting the heat shock protein (groEL) gene and the major surface protein4 gene (msp4), A. capra was detected from 129 (8.9%) of 1453 sheep and goat blood samples. The positive rate was higher in goats (9.4%, 89/943) than in sheep (7.8%, 40/510) (2 = 1.04, p > 0.05, df=1). For sheep, A. capra was found in 17 sites from 2 provinces. The prevalence was 28.6% in sheep from Liaoning province, which was higher than in Henan Province (7.3%). For goats, A. capra was detected in 35 sites from 7 provinces. The prevalence varied from 0% to 19.4% in the goat sites examined. The prevalences were 19.4%,19.3%, 10%, 8.8%, 6.8%, 1.8% and 0% in goats from Guizhou province, Henan Province, Inner Mongolia Autonomous Region, Shanxi Province, Xinjiang Uygur Autonomous Region, Yunnan province and Gansu province respectively. Based on the analysis of the A. capra citrate synthase gene (gltA), two variants were identified. Variant I showed high sequence similarity to those A. capra, which were previously reported in sheep, goats, Ixodes persulcatus, Haemaphysalis longicornis, Haemaphysalis qinghaiensis, and human. Variant II was only found in Luoyang, Anyang, and Sanmengxia, of Henan province. To our knowledge, this is the first detection of this variant of A. capra in sheep and goat blood in China. Phylogenetic analysis based on groEL and msp4 genes showed that the Anaplasma sp. sequences clustered independently from A. capra and other Anaplasma species with high bootstrap values. We found A. capra DNA in sheep and goats in China, providing evidence that sheep and goats can be infected by A. capra. We also found that this zoonotic pathogen is widely distributed in China. This study provides information for assessing the public health risks for human anaplasmosis.