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Comprehensive innate immune profiling of chikungunya virus infection in pediatric cases

2018; Springer Nature; Volume: 14; Issue: 8 Linguagem: Inglês

10.15252/msb.20177862

1744-4292

Daniela Michlmayr, Theodore Pak, Adeeb Rahman, El-ad David Amir, Eun‐Young Kim, Seunghee Kim‐Schulze, Maria Suprun, Michael G. Stewart, S Wehrlen-Pugliese, Ángel Balmaseda, Li Wang, Jun Zhu, Mayte Suárez‐Fariñas, Steven M. Wolinsky, Andrew Kasarskis, Eva Harris,

Respiratory viral infections research

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes global epidemics of debilitating disease worldwide. To gain functional insight into the host cellular genes required for virus infection, we performed whole-blood RNA-seq, 37-plex mass cytometry of peripheral blood mononuclear cells (PBMCs), and serum cytokine measurements of acute- and convalescent-phase samples obtained from 42 children naturally infected with CHIKV Semi-supervised classification and clustering of single-cell events into 57 sub-communities of canonical leukocyte phenotypes revealed a monocyte-driven response to acute infection, with the greatest expansions in "intermediate" CD14