Produção Nacional
Revisado por pares
Parallel damage in mitochondria and lysosomes is an efficient way to photoinduce cell death
2018; Taylor & Francis; Volume: 15; Issue: 2 Linguagem: Inglês
10.1080/15548627.2018.1515609
ISSN1554-8635
AutoresWaleska K. Martins, Nayra Fernandes Santos, Cleidiane de Sousa Rocha, Isabel O. L. Bacellar, Tayana Mazin Tsubone, Ana Cláudia Viotto, Adriana Yamaguti Matsukuma, Aline B. de P. Abrantes, Paulo Siani, Luís Gustavo Dias, Maurı́cio S. Baptista,
Tópico(s)Redox biology and oxidative stress
ResumoCells challenged by photosensitized oxidations face strong redox stresses and rely on autophagy to either survive or die. However, the use of macroautophagy/autophagy to improve the efficiency of photosensitizers, in terms of inducing cell death, remains unexplored. Here, we addressed the concept that a parallel damage in the membranes of mitochondria and lysosomes leads to a scenario of autophagy malfunction that can greatly improve the efficiency of the photosensitizer to cause cell death. Specific damage to these organelles was induced by irradiation of cells pretreated with 2 phenothiazinium salts, methylene blue (MB) and 1,9-dimethyl methylene blue (DMMB). At a low concentration level (10 nM), only DMMB could induce mitochondrial damage, leading to mitophagy activation, which did not progress to completion because of the parallel damage in lysosome, triggering cell death. MB-induced photodamage was perceived almost instantaneously after irradiation, in response to a massive and nonspecific oxidative stress at a higher concentration range (2 µM). We showed that the parallel damage in mitochondria and lysosomes activates and inhibits mitophagy, leading to a late and more efficient cell death, offering significant advantage (2 orders of magnitude) over photosensitizers that cause unspecific oxidative stress. We are confident that this concept can be used to develop better light-activated drugs.
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