Portal de Periódicos da CAPES

An In Vitro Model for Identifying Cardiac Side Effects of Anesthetics

2018; Lippincott Williams & Wilkins; Volume: 130; Issue: 1 Linguagem: Inglês

10.1213/ane.0000000000003757

1526-7598

Alex Chia Yu Chang, Andrew Chia Hao Chang, Luka Nicin, Gerhard Weber, Colin Holbrook, M. Frances Davies, Helen M. Blau, Edward Bertaccini,

Pluripotent Stem Cells Research

The understanding of anesthetic side effects on the heart has been hindered by the lack of sophisticated clinical models. Using micropatterned human-induced pluripotent stem cell–derived cardiomyocytes, we obtained cardiac muscle depressant profiles for propofol, etomidate, and our newly identified anesthetic compound KSEB01-S2. Propofol was the strongest depressant among the 3 compounds tested, exhibiting the largest decrease in contraction velocity, depression rate, and beating frequency. Interestingly, KSEB01-S2 behaved similarly to etomidate, suggesting a better cardiac safety profile. Our results provide a proof-of-concept for using human-induced pluripotent stem cell–derived cardiomyocytes as an in vitro platform for future drug design.