Surface Engineering of the RNA Coliphage Q? to Display Plasmodium Falciparum Derived Asexual Blood Stage Antigens UB05 and Merozoite Surface Protein 3
2018; OMICS Publishing Group; Volume: 07; Issue: 04 Linguagem: Inglês
10.4172/2327-5073.1000314
ISSN2327-5073
AutoresAlain Bopda Waffo, Abel Lissom, Herve Fotso Ouambo, Loveline Ngu, Apeh A. Ngoh, Carrie Sanders, Swapnil Bawage, Colince J. Tchadji, Jules N C Assob, Arinze S. Okoli, Doline Takoua, Georgia Ambada, Ghislain Donald Njambe Priso, Rosario García, Anna Gutiérrez, Dieudonné Ndjonka, George O Chukwuma, Malachy Ifeanyi Okeke, Rosette Megnekou, Charles Okechukwu Esimone, Eric Achidi, Wilfred N Mbacham, Lazare Kaptué, Chae Gyu Park, Godwin Nchinda,
Tópico(s)Mosquito-borne diseases and control
ResumoBackground: Naturally acquired immune responses to Plasmodium falciparum merozoite surface protein 3 (MSP3) and UB05 are implicated in semi immunity in populations living in malaria endemic areas.Thus designing chimeric malaria vaccine candidates involving MSP-3 and UB05 displayed upon the surface of a phage in its native form could potentiate their immunogenicity and antigenicity.In this study, we have engineered both MSP3 and UB05 upon the Qβ and assessed their antigenicity with plasma from children living in a high malaria transmission region of Cameroon. Methods:The surface of the RNA coliphage Qβ was genetically modified to display three Plasmodium falciparum derived immunogens including MSP3, UB05 and a chimera of the two UB05-MSP3.The resultant recombinant phages including QβMSP3, QβUB05 and QβUB05-MSP3 with surface displayed malaria immunogens were produced after transformation of the E. coli strain HB101.Plasma levels of antigen specific IgG antibody were then determined in samples from malaria positive and negative children living in a high malaria transmission region of Cameroon. Results:To improve yield each recombinant phage was scaled up to 10 14 pfu/ml using production strategies previously optimized in our group.This was significantly higher (P<0.001)relative to the 10 8 pfu/ml of the wild type C li
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