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Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis

2018; Cell Press; Volume: 24; Issue: 12 Linguagem: Inglês

10.1016/j.celrep.2018.08.057

2639-1856

Yu Fujita, Ali Khateb, Yan Li, Roberto Tinoco, Tongwu Zhang, Haggai Bar‐Yoseph, Miguel A. Tam, Yehuda Chowers, Edmond Sabo, Shiran Gerassy-Vainberg, Elina Starosvetsky, Brian P. James, Kevin M. Brown, Shai S. Shen-Orr, Linda M. Bradley, Philippe A. Tessier, Ze’ev A. Ronai,

Neonatal Respiratory Health Research

Inflammatory bowel disease (IBD) is prevalent, but the mechanisms underlying disease development remain elusive. We identify a role for the E3 ubiquitin ligase RNF5 in IBD. Intestinal epithelial cells (IECs) express a high level of RNF5, while the colon of Rnf5-/- mice exhibits activated dendritic cells and intrinsic inflammation. Rnf5-/- mice exhibit severe acute colitis following dextran sodium sulfate (DSS) treatment. S100A8 is identified as an RNF5 substrate, resulting in S100A8 ubiquitination and proteasomal-dependent degradation that is attenuated upon inflammatory stimuli. Loss of RNF5 from IECs leads to enhanced S100A8 secretion, which induces mucosal CD4+ T cells, resulting in Th1 pro-inflammatory responses. Administration of S100A8-neutralizing antibodies to DSS-treated Rnf5-/- mice attenuates acute colitis development and increases survival. An inverse correlation between RNF5 and S100A8 protein expression in IECs of IBD patients coincides with disease severity. Collectively, RNF5-mediated regulation of S100A8 stability in IECs is required for the maintenance of intestinal homeostasis.