Portal de Periódicos da CAPES

Comparison of selected CD45+ cell subsets’ response and cytokine levels on exhaustive effort among soccer players

2018; De Gruyter; Volume: 38; Issue: 3 Linguagem: Inglês

10.2478/jomb-2018-0029

1452-8258

Dorota Kostrzewa‐Nowak, Rafał Buryta, Robert Nowak,

Muscle metabolism and nutrition

Immunological alterations may led to the reduction in capacity and endurance levels in elite athletes by e.g. increased susceptibility to infections. There is a need to explain the impact of intensive physical effort on the CD4+ memory T cell subsets.Fourteen participants median aged 19 years old (range 17-21 years) were recruited form Pogoń Szczecin S.A., soccer club. They performed progressive efficiency test on mechanical treadmill until exhaustion twice: during preparatory phases to spring and autumn competition rounds. We examined the influence of exhaustive effort on the selected CD45+, especially CD4+ memory T cell subsets and inflammation markers determined before, just after the test and during recovery time.Significant changes in total CD45+ cells and decrease in T lymphocytes percentage after the run was observed. Significant fluctuations in T cells' distribution were related not only to the changes in Th or Tc subsets but also to increase in naïve T cell percentage during recovery. Increase in TNF-α and IL-8 post-exercise, IL-6 and IL-10 plasma levels in recovery was also found.The novel finding of our study is that the run performed on mechanical treadmill caused a significant release of CD4+ T naïve cells into circulation. Post-exercise increase in circulating NK cells is related with fast biological response to maximal effort. However, at the same time an alternative mechanism enhancing inflammation is involved.Imunološke promene mogu da dovedu do umanjenja kapaciteta i nivoa otpornosti elitnih atletičara npr. usled povećane osetljivosti na infekcije. Zato je potrebno ispitati uticaj intenzivnog fizičkog napora na memoriju T ćelija CD4+.Četrdeset učesnika fudbalskog kluba Pago Szezecin S.A. starosti srednjih godina 19 (između 17–21 godina) ipitivani su u ovom radu. Oni su izvodili progresivni test efikanosti sve do izrazitog napora dva puta: za vreme priprema u toku proleća i u toku jesenje runde. Ispitivan je uticaj takvog zamaranja na CD45+, i to specijalno na memorije T ćelije CD4+, kao i na inflamacione markere određene pre, kao i nakon testa i u periodu oporavka.Uočene su značajne promene u ukupnim CD4+ ćelijama i procenta T limfocita nakon trčanja. Značajne fluktuacije u raspodeli T ćelija nisu bile samo povezane sa promenama Th ili Tc, već i povećanjem procenta T ćelija u toku oporavka. Nađeno je i povećanje nivoa u plazmi TNF-α i IL-8 nakon vežbanja, IL-6 i IL-10 nakon oporavka.U ovom istraživanju nađeno je značajno povećanje CD4+ T ćelija u cirkulaciji usled mehaničkog napora. Povećanje cirkulišućih NK ćelija u post-fazi vežbanja objašnjava se brzim biološkim odgovorom na maksimalni napor. Međutim, istovremeno se uključuje i alternativni mehanizam koji dovodi do povećanja inflamacije.