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Expansion and Phenotypic Changes of Mouse Bone Marrow Mesenchymal Cells Cultured with FGF-2 and Facial Nerve-Conditioned Medium

2018; Chilean Society of Anatomy; Volume: 36; Issue: 3 Linguagem: Inglês

10.4067/s0717-95022018000301049

0717-9502

Eudes Euler de Souza Lucena, Hécio Henrique Araújo de Morais, Dayane Pessoa de Araújo, José Rodolfo Lopes de Paiva Cavalcanti, Eduardo Pereira de Azevedo, Dinalva Brito de Queiróz, Marco Antônio Botelho, Amália Cínthia Meneses do Rêgo, Irami Araújo-Filho, Carlos Augusto Galvão Barboza, Expedito Silva do Nascimento, Miriam Stela Maris de Oliveira Costa, Jeferson de Sousa Cavalcante, Fausto Pierdoná Gúzen,

Wound Healing and Treatments

Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation.