First Report of Anthracnose Crown Rot of Strawberry Caused by Colletotrichum siamense in Rajshahi District of Bangladesh
2018; American Phytopathological Society; Volume: 103; Issue: 3 Linguagem: Inglês
10.1094/pdis-08-18-1461-pdn
ISSN1943-7692
AutoresDipali Rani Gupta, M. K. Kabir, Oliul Hassan, Abdullah As Sabir, Nur Uddin Mahmud, Musrat Zahan Surovy, Mahfuzur Rahman, Taehyun Chang, Daniel G. Panaccione, Tofazzal Islam,
Tópico(s)Banana Cultivation and Research
ResumoHomePlant DiseaseVol. 103, No. 3First Report of Anthracnose Crown Rot of Strawberry Caused by Colletotrichum siamense in Rajshahi District of Bangladesh PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Anthracnose Crown Rot of Strawberry Caused by Colletotrichum siamense in Rajshahi District of BangladeshD. R. Gupta, M. K. Kabir, O. Hassan, A. A. Sabir, N. U. Mahmud, M. Z. Surovy, M. M. Rahman, T. Chang, D. G. Panaccione, and M. T. IslamD. R. GuptaSearch for more papers by this author, M. K. KabirSearch for more papers by this author, O. Hassanhttp://orcid.org/0000-0001-9514-4581Search for more papers by this author, A. A. SabirSearch for more papers by this author, N. U. MahmudSearch for more papers by this author, M. Z. SurovySearch for more papers by this author, M. M. RahmanSearch for more papers by this author, T. Changhttp://orcid.org/0000-0002-0213-7444Search for more papers by this author, D. G. PanaccioneSearch for more papers by this author, and M. T. Islam†Corresponding author: M. T. Islam; E-mail: E-mail Address: tofazzalislam@yahoo.comhttp://orcid.org/0000-0002-7613-0261Search for more papers by this authorAffiliationsAuthors and Affiliations D. R. Gupta M. K. Kabir , Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh O. Hassan , Department of Ecology and Environmental System, College of Ecology and Environmental Sciences, Kyungpook National University, Sangju, Gyeongbuk 37224, Republic of Korea A. A. Sabir N. U. Mahmud M. Z. Surovy , Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh M. M. Rahman , West Virginia University, Morgantown, WV, U.S.A. T. Chang , Department of Ecology and Environmental System, College of Ecology and Environmental Sciences, Kyungpook National University, Sangju, Gyeongbuk 37224, Republic of Korea D. G. Panaccione , West Virginia University, Morgantown, WV, U.S.A. M. T. Islam † , Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh; and West Virginia University, Morgantown, WV, U.S.A. Published Online:31 Dec 2018https://doi.org/10.1094/PDIS-08-18-1461-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Strawberry (Fragaria × ananassa) was introduced to Bangladesh in 1996 and has gained remarkable acceptance. However, with the expansion of acreage, anthracnose crown rot (ACR) is posing a challenge to strawberry growers. In March 2016, typical ACR symptoms were observed on cultivar Strawberry Festival in a farmer’s field in Rajshahi District. Symptoms were evident on approximately 10% of plants and included flagging of young leaves, mimicking lack of water, and wilting of the entire plant in daytime heat. A lengthwise cut through the crown of diseased plants showed characteristic shades of red and white marbling similar to that described by Weir et al. (2012). Crowns of seven symptomatic strawberry plants were obtained after excising foliage and roots and washed under running water for 1 h. Crowns were then surface disinfested with 70% ethanol followed by 1% NaClO for 2 and 3 min, respectively. Small pieces (2 × 2 mm) of the internal infected marbled red tissues were cut with a sterile scalpel, placed on corn meal agar, and incubated for 7 days at 25°C. Conidia grown on tissue pieces and hyphal mats from the tissues were streaked on PDA plates to obtain single conidial colonies. Two of the single-conidial isolates (BRSP08 and BRSP09) that had white margins with circular, dull orange centers were selected to prove Koch’s postulates. The average mycelial growth rate was 9.8 mm/day at 25°C on PDA with cottony aerial growth and pale yellowish to pinkish color. Conidia were hyaline, cylindrical, with round ends, and measuring 14.90 to 18.19 × 4.05 to 6.20 μm (average ± SD, 16.22 ± 1.20 × 5.90 ± 0.84 μm) (n = 30). Appressoria were brown, globose to cylindrical, lobbed, and measuring 7.50 to 12.70 × 6.08 to 8.4 μm (10.72 ± 1.30 × 7.20 ± 0.80 μm) (n = 20). The morphological characteristics of these isolates are consistent with some Colletotrichum species within the C. gloeosporioides complex including C. siamense (Afanador-Kafuri et al. 2003; Weir et al. 2012). BRSP09 was used for molecular identification. Internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS) and genes encoding chitin synthase (CHS-1), actin (ACT), β-tubulin (TUB2), calmodulin (CAL), and the Apn2–Mat1–2 intergenic spacer and partial mating type (Mat1–2) gene (ApMat) of the selected isolate were amplified using primers ITS1F/ITS4, Bt2a/Bt-2b, CHS-79F/CHS-354R, ACT512F/ACT783R, CL1C/CL2C, and AM-F/AM-R, respectively (Silva et al. 2012; Weir et al. 2012). Sequencing of the purified polymerase chain reaction products was performed by Macrogen (Korea), and sequences were deposited in GenBank (LC420017 to LC420022). Neighbor-joining and maximum likelihood phylogenetic trees were inferred using MEGA 6.0 (Tamura et al. 2013) based on the multilocus alignment (ITS, TUB2, ACT, CHS-1, CAL, and ApMat) of the present isolate and related Colletotrichum spp. sequences previously deposited in GenBank. BLAST search and phylogenetic analysis revealed that BRSP09 is C. siamense (Mills et al. 1992). To confirm the pathogenicity of the fungus, ten 3-month-old tissue-cultured Strawberry Festival plants were inoculated by dipping the roots and crowns into either 300 ml of conidial suspension (8 × 106 conidia/ml) of the isolates BRSP08 and BRSP09 or sterile distilled water (control) for 30 min. The inoculated plants were transplanted into plastic pots with artificial peat-based soil mix and maintained in the greenhouse with 28/20°C day/night temperature. The experiment was conducted twice. After 4 weeks, all plants inoculated with BRSP08 or BRSP09 were stunted and developed typical wilt symptoms similar to those observed in the field, whereas the control plants remained healthy. C. siamense was reisolated from crown tissues with typical ACR symptoms and identified using morphological and molecular methods mentioned above, fulfilling Koch’s postulates. This is the first report of ACR of strawberry caused by C. siamense and the members of C. siamense complex in Rajshahi District.References:Afanador-Kafuri, L., et al. 2003. Phytopathology 93:579. https://doi.org/10.1094/PHYTO.2003.93.5.579 Link, ISI, Google ScholarMills, P. R., et al. 1992. FEMS Microbiol. Lett. 98:137. https://doi.org/10.1111/j.1574-6968.1992.tb05503.x Crossref, ISI, Google ScholarSilva, D. N., et al. 2012. Mycologia 104:396. https://doi.org/10.3852/11-145 Crossref, ISI, Google ScholarTamura, K., et al. 2013. Mol. Biol. Evol. 30:2725. https://doi.org/10.1093/molbev/mst197 Crossref, ISI, Google ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarD. R. Gupta, M. K. Kabir, O. Hassan, and A. A. Sabir contributed equally to this work.Funding: This research was partially funded by the Government of Bangladesh and World Bank through HEQEP CPSF 2071 to M. T. Islam of Department of Biotechnology of BSMRAU, and by the USDA Foreign Agricultural Service program contract Agr number SR-CR-13–002. The James William Fulbright Visiting Scholar Fellowship from the U.S. Government to M. T. Islam is also highly acknowledged.DetailsFiguresLiterature CitedRelated Vol. 103, No. 3 March 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 6 Mar 2019Published: 31 Dec 2018First Look: 20 Sep 2018Accepted: 19 Sep 2018 Pages: 580-580 Information© 2019 The American Phytopathological SocietyFundingGovernment of Bangladesh and World BankGrant/Award Number: HEQEP CPSF 2071Foreign Agricultural ServiceGrant/Award Number: Agr number SR-CR-13–002Cited byColletotrichum species pathogenic to strawberry: discovery history, global diversity, prevalence in China, and the host range of top two species16 November 2022 | Phytopathology Research, Vol. 4, No. 1Colletotrichum siamenseCABI Compendium, Vol. CABI CompendiumDiversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort17 November 2021 | Frontiers in Microbiology, Vol. 12Fabrication of highly and poorly oxidized silver oxide/silver/tin(IV) oxide nanocomposites and their comparative anti-pathogenic properties towards hazardous food pathogensJournal of Hazardous Materials, Vol. 408First Report of Anthracnose Causing Both Crown and Fruit Rot of Strawberry by Colletotrichum siamense in North CarolinaT. 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