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Revisado por pares
First Report of Neoscytalidium dimidiatum Causing Root Rot in Sweet Potato in Brazil
2018; American Phytopathological Society; Volume: 103; Issue: 2 Linguagem: Inglês
10.1094/pdis-07-18-1242-pdn
ISSN1943-7692
AutoresJuliana Ferreira de Mello, Amanda Cupertino de Queiroz Brito, Cristina Maria de Souza‐Motta, Janiele Cássia Barbosa Vieira, Sami Jorge Michereff, Alexandre Reis Machado,
Tópico(s)Plant Disease Management Techniques
ResumoHomePlant DiseaseVol. 103, No. 2First Report of Neoscytalidium dimidiatum Causing Root Rot in Sweet Potato in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Neoscytalidium dimidiatum Causing Root Rot in Sweet Potato in BrazilJ. F. Mello, A. C. Q. Brito, C. M. S. Motta, J. C. B. Vieira, S. J. Michereff, and A. R. MachadoJ. F. Mello, A. C. Q. Brito, C. M. S. Motta, J. C. B. Vieira, S. J. Michereff, and A. R. Machado†Corresponding author: A. R. Machado; E-mail: E-mail Address: [email protected]http://orcid.org/0000-0001-7440-3097AffiliationsAuthors and Affiliations J. F. Mello A. C. Q. Brito C. M. S. Motta , Departamento de Micologia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil J. C. B. Vieira , Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil S. J. Michereff , Centro de Ciências Agrárias e da Biodiversidade, Universidade Federal do Cariri, Crato, Ceará, 63130-025, Brazil A. R. Machado † , Departamento de Micologia, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-600, Brazil. Published Online:17 Dec 2018https://doi.org/10.1094/PDIS-07-18-1242-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In January 2018, a large number of tuberous roots of sweet potato were lost in a production field in Jaíba, State of Minas Gerais, Brazil. Immediately after harvest, the roots showed dark spots on the surface, extending to the center, causing an internal, brown to black necrosis. Two symptomatic roots (cultivar Canadense) from different parts of the field were subjected to fungus isolation by removal of fragments between symptomatic and healthy tissue, followed by surface sterilization in 70% ethanol and then in sodium hypochlorite (1%) for 1 and 3 min, respectively, rinsing in sterile water, and incubation in PDA plates for 25°C in the dark. A gray to black, fast-growing fungus with abundant arthroconidia in aerial mycelia was isolated from all samples after 4 days of incubation. Hyphal tips were excised and transferred to new PDA plates, and two pure cultures (isolates) were obtained. Based on these characteristics, the fungus was identified as Neoscytalidium. For morphological analysis, culture sporulation was induced as described by Machado et al. (2014). Microscopic examination of the fungal structures identified arthroconidia with one to two septa, released by hyphal fragmentation, 4.2 to 9.5 × 3.2 to 5.3 μm, and a pycnidial synanamorph with hyaline, ellipsoid to nearly fusiform conidia, 7.4 to 12.7 × 4.2 to 6.3 μm. The morphological characteristics were similar to Neoscytalidium dimidiatum (Phillips et al. 2013). To confirm species identification, genomic DNA was extracted, and sequences of the internal transcribed spacer of the rDNA and translation elongation factor 1-α (TEF1-α) were obtained (Machado et al. 2014) and deposited in GenBank (accession nos. MH636327, MH636328, MH349427, and MH349428, respectively). According to a BLAST search, the TEF1-α sequences showed 99% identity with N. dimidiatum (accession no. KF553902). A combined phylogenetic tree obtained by Bayesian inference using MrBayes version 3.1.1 (Ronquist and Huelsenbeck 2003) grouped the isolates within the N. dimidiatum clade, confirming the species identification. One representative isolate (URM 7888) was deposited in the culture collection "Micoteca URM Profa. Maria Auxiliadora Cavalcanti" at the Universidade Federal de Pernambuco (Recife, Brazil). The pathogenicity test was performed by inoculating asymptomatic roots (cultivar Canadense), previously sterilized on the surface with 0.5% sodium hypochlorite and unwounded or wounded with a sterile scalpel at two equidistant points, with conidia suspension. Arthroconidia suspension (20 μl, 2.4 × 106 conidia/ml) obtained from 7-day-old colonies was pipetted onto the inoculation sites. Sterile distilled water was used for the control instead of inoculum. Five roots were used for each treatment. The inoculated roots were maintained in plastic boxes containing moistened paper towels at approximately 25°C for 15 days. After 7 days, it was possible to observe initial symptoms of internal brown to black rot, which increased in size after 15 days. The fungus was reisolated, and pathogenicity was confirmed. The control and unwounded roots remained asymptomatic. In Brazil, N. dimidiatum has been reported causing collar and root rot in physic nut (Machado et al. 2012), black root rot in cassava (Machado et. al., 2014), and dieback in mango (Marques et al. 2013). To our knowledge, this is the first report of N. dimidiatum causing root rot of sweet potato in Brazil.References:Machado, A. R., et al. 2012. Plant Dis. 96:1697. https://doi.org/10.1094/PDIS-05-12-0504-PDN Link, ISI, Google ScholarMachado, A. R., et al. 2014. Trop. Plant Pathol. 39:464. https://doi.org/10.1590/S1982-56762014000600008 Crossref, ISI, Google ScholarMarques, M. W., et al. 2013. Fungal Divers. 61:195. https://doi.org/10.1007/s13225-013-0258-1 Crossref, ISI, Google ScholarPhillips, A. J. L., et al. 2013. Stud. Mycol. 76:51. https://doi.org/10.3114/sim0021 Crossref, ISI, Google ScholarRonquist, F., and Huelsenbeck, J. P. 2003. Bioinformatics 19:1572. https://doi.org/10.1093/bioinformatics/btg180 Crossref, ISI, Google ScholarFunding: Funding was provided by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco, and Conselho Nacional de Desenvolvimento Científico e Tecnológico.DetailsFiguresLiterature CitedRelated Vol. 103, No. 2 February 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 24 Jan 2019Published: 17 Dec 2018First Look: 18 Sep 2018Accepted: 16 Sep 2018 Page: 373 Information© 2019 The American Phytopathological SocietyFundingCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFundação de Amparo à Ciência e Tecnologia do Estado de PernambucoConselho Nacional de Desenvolvimento Científico e TecnológicoCited byFirst report of Nigrospora vesicularifera causing foot rot on sweet potato in BrazilCrop Protection, Vol. 166Morphological and molecular characterization of Neoscytalidium isolates that cause canker and dieback in Eucalyptus and Chinaberry trees in IraqPlant Protection Science, Vol. 59, No. 1Neoscytalidium novaehollandiae causes dieback on Pinus eldarica and its potential for infection of urban forest trees4 June 2022 | Scientific Reports, Vol. 12, No. 1A review of research trends on Nattrassia mangiferae from 1966-2022: A bibliometric approach30 November 2022 | Scientific Research and Essays, Vol. 17, No. 4Morphological, Molecular Identification and Pathogenicity of Neoscytalidium dimidiatum Causing Stem Canker of Hylocereus polyrhizus in Southern Thailand12 February 2022 | Plants, Vol. 11, No. 4Neoscytalidium dimidiatumCABI Compendium, Vol. CABI CompendiumIdentification and pathogenicity of Botryosphaeriaceae species associated with root and stem rot of sweet potato in Brazil24 May 2021 | Plant Pathology, Vol. 70, No. 7Neoscytalidium dimidiatum causes leaf blight on Sansevieria trifasciata in Brazil23 April 2020 | Australasian Plant Disease Notes, Vol. 15, No. 1Bioprospecting for secondary metabolites of family Botryosphaeriaceae from a biotechnological perspective
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