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Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo

2018; Cell Press; Volume: 24; Issue: 13 Linguagem: Inglês

10.1016/j.celrep.2018.08.088

2639-1856

Leiron Ferrarese, Jean-Sébastien Jouhanneau, Michiel W. H. Remme, Jens Kremkow, Gergely Katona, Balázs Rózsa, Susanne Schreiber, James F.A. Poulet,

Neuroscience and Neural Engineering

Excitatory synaptic input reaches the soma of a cortical excitatory pyramidal neuron via anatomically segregated apical and basal dendrites. In vivo, dendritic inputs are integrated during depolarized network activity, but how network activity affects apical and basal inputs is not understood. Using subcellular two-photon stimulation of Channelrhodopsin2-expressing layer 2/3 pyramidal neurons in somatosensory cortex, nucleus-specific thalamic optogenetic stimulation, and paired recordings, we show that slow, depolarized network activity amplifies small-amplitude synaptic inputs targeted to basal dendrites but reduces the amplitude of all inputs from apical dendrites and the cell soma. Intracellular pharmacology and mathematical modeling suggests that the amplification of weak basal inputs is mediated by postsynaptic voltage-gated channels. Thus, network activity dynamically reconfigures the relative somatic contribution of apical and basal inputs and could act to enhance the detectability of weak synaptic inputs.