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Bioconversion of plant biomass hydrolysate into bioplastic (polyhydroxyalkanoates) using Ralstonia eutropha 5119

2018; Elsevier BV; Volume: 271; Linguagem: Inglês

10.1016/j.biortech.2018.09.122

1873-2976

Shashi Kant Bhatia, Ranjit Gurav, Tae‐Rim Choi, Hye‐Rim Jung, Soo‐Yeon Yang, Yu‐Mi Moon, Hun‐Suk Song, Jong-Min Jeon, Kwon‐Young Choi, Yung‐Hun Yang,

Catalysis for Biomass Conversion

Pretreatment of lignocellulosic biomass results in the formation of byproducts (furfural, hydroxymethylfurfural [HMF], vanillin, acetate etc.), which affect microbial growth and productivity. Furfural (0.02%), HMF (0.04%), and acetate (0.6%) showed positive effects on Ralstonia eutropha 5119 growth and polyhydroxyalkanoate (PHA) production, while vanillin exhibited negative effects. Response optimization and interaction studies between the variables glucose, ammonium chloride, furfural, HMF, and acetate using the response surface methodology resulted in maximum PHA production (2.1 g/L) at optimal variable values of 15.3 g/L, 0.43 g/L, 0.04 g/L, 0.05 g/L, and 2.34 g/L, respectively. Different lignocellulosic biomass hydrolysates (LBHs), including barley biomass hydrolysate (BBH), Miscanthus biomass hydrolysate (MBH), and pine biomass hydrolysate (PBH), were evaluated as potential carbon sources for R. eutropha 5119 and resulted in 1.8, 2.0, and 1.7 g/L PHA production, respectively. MBH proved the best carbon source, resulted in higher biomass (Yx/s, 0.31 g/g) and PHA (Yp/s, 0.14 g/g) yield.