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First Report of Colletotrichum tropicale Causing Anthracnose on Pomegranate in Brazil
2018; American Phytopathological Society; Volume: 103; Issue: 3 Linguagem: Inglês
10.1094/pdis-05-18-0767-pdn
ISSN1943-7692
AutoresJ. R. A. Silva-Cabral, Lourdes Regina Lopes Batista, Jaqueline Figueredo de Oliveira Costa, Mayra Machado de Medeiros Ferro, S. J. C. Silva, Gaus Silvestre de Andrade Lima, Iraíldes Pereira Assunção,
Tópico(s)Pomegranate: compositions and health benefits
ResumoHomePlant DiseaseVol. 103, No. 3First Report of Colletotrichum tropicale Causing Anthracnose on Pomegranate in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Colletotrichum tropicale Causing Anthracnose on Pomegranate in BrazilJ. R. A. Silva-Cabral, L. R. L. Batista, J. F. de O. Costa, M. M. de M. Ferro, S. J. C. Silva, G. S. de A. Lima, and I. P. AssunçãoJ. R. A. Silva-Cabral†Corresponding author: J. R. A. Silva-Cabral; E-mail: E-mail Address: [email protected], L. R. L. Batista, J. F. de O. Costa, M. M. de M. Ferro, S. J. C. Silvahttp://orcid.org/0000-0001-5878-3077, G. S. de A. Lima, and I. P. AssunçãoAffiliationsAuthors and Affiliations J. R. A. Silva-Cabral † L. R. L. Batista J. F. de O. Costa M. M. de M. Ferro S. J. C. Silva G. S. de A. Lima I. P. Assunção , Setor de Fitossanidade/Centro de Ciências Agrárias, Universidade Federal de Alagoas, Rio Largo, AL, Brazil, 57100-000. Published Online:17 Jan 2019https://doi.org/10.1094/PDIS-05-18-0767-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Anthracnose is a common disease that affects yield and quality of pomegranate (Punica granatum L.) fruit in different parts of world including Brazil. Colletotrichum gloeosporioides Penz. was considered to be the causal agent of pomegranate anthracnose because the identification of this species only relied on morphological characteristics and analysis of the ribosomal internal transcribed spacer region (ITS) (Rahimlou et al. 2014). Recently, multigene phylogenetic analyses revealed that C. gloeosporioides is a species complex (Weir et al. 2012). With an ApMat-based phylogeny, C. theobromicola Delacr. and an unresolved taxon were isolated from anthracnose lesions on pomegranate fruits (Sharma et al. 2015). Fruits of pomegranate showing dark, circular, and necrotic lesions were collected in the states of Alagoas and Bahia, in the northeastern region of Brazil, between August 2012 and January 2013. Small pieces of tissue taken from the lesions were surface disinfested in 70% ethanol for 30 s and in 1% NaClO for 1 min, rinsed in sterile water, plated on potato dextrose agar (PDA; Kasvi), and incubated at 25°C for 7 days. After 7 days on PDA at 25°C, colonies of the isolates varied between white and gray with a greenish reverse, and with a mean growth rate of 7.6 mm/day. Conidia were hyaline, cylindrical with rounded ends, and measured 14.3 (12.30 to 20.01) × 4.8 (3.36 to 6.11) μm. Appressoria were brown, clavate, and globose and measured 8.4 (5.28 to 12.12) × 6.3 (4.8 to 9.1) µm, which matched with those described for C. tropicale. Three single-spore isolates were obtained and stored in the Culture Collection of Phytopathogenic Fungi at the Universidade Federal de Alagoas. Genomic DNA was used as template for polymerase chain reaction amplifications of partial sequences of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-tubulin (TUB2) genes and the ITS region (Weir et al. 2012). In the GenBank database, sequences of GAPDH (97 to 100%) and TUB2 (100%) were similar to those of C. tropicale E.I. Rojas, S.A. Rehner & Samuels, whereas those of ITS were 99 to 100% similar with C. siamense, C. tropicale, and C. gloeosporioides, all members of the C. gloeosporioides complex. Phylogenetic Bayesian inference analysis based in a combined data set (GAPDH, TUB2, and ITS) confirmed our isolates as C. tropicale. Partial sequences were deposited in GenBank (KY769889, KY769899, KY769905, KY769890, KY769891, MG647010, and MG647012), and the alignment and tree were deposited into TreeBASE (https://www.treebase.org/; accession no. 22650). To confirm the pathogenicity, 5-mm mycelial discs from a 7-day-old PDA culture were placed on healthy pomegranate fruits superficially wounded with a sterilized needle. In control fruits, only PDA discs were used. Fruits were kept in a humid chamber at 25°C. After 7 days, circular necrotic lesions were observed on inoculated fruits. To fulfill Koch's postulates, C. tropicale was successfully reisolated from inoculated fruits. The control fruits showed no symptoms. Although C. tropicale was previously isolated from fruits of carnauba palm and mango in Brazil (Araújo et al. 2018; Lima et al. 2013), this is its first report on pomegranate fruits in the world.References:Araújo, M. B. M., et al. 2018. Plant Dis. 102:244. https://doi.org/10.1094/PDIS-06-17-0860-PDN Link, Google ScholarLima, N. B., et al. 2013. Fungal Divers. 61:75. https://doi.org/10.1007/s13225-013-0237-6 Crossref, ISI, Google ScholarRahimlou, S., et al. 2014. J. Plant Pathol. 96:603. Google ScholarSharma, G. et al. 2015. Fungal Divers. 71:247. https://doi.org/10.1007/s13225-014-0312-7 Crossref, ISI, Google ScholarWeir, P. F., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarFunding: Funding was provided by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa do Estado de Alagoas (FAPEAL).DetailsFiguresLiterature CitedRelated Vol. 103, No. 3 March 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 6 Mar 2019Published: 17 Jan 2019First Look: 2 Oct 2018Accepted: 29 Sep 2018 Pages: 583-583 Information© 2019 The American Phytopathological SocietyFundingCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de Alagoas (FAPEAL)Cited byPomegranate: Postharvest Fungal Diseases and Control3 March 2023Postharvest Rot of Pomegranate Fruit in Southern Italy: Characterization of the Main Pathogens30 April 2022 | Journal of Fungi, Vol. 8, No. 5Colletotrichum species associated with sugarcane red rot in BrazilFungal Biology, Vol. 126, No. 4First report of Colletotrichum tropicale on dragon fruit and the response of three Selenicereus species to anthracnose17 January 2022 | International Journal of Pest Management, Vol. 103Colletotrichum tropicaleCABI Compendium, Vol. CABI CompendiumColletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1Characterization of Colletotrichum Species Causing Anthracnose of Pomegranate in the Southeastern United StatesKatia V. 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