Portal de Periódicos da CAPES

PHLI-seq: constructing and visualizing cancer genomic maps in 3D by phenotype-based high-throughput laser-aided isolation and sequencing

2018; BioMed Central; Volume: 19; Issue: 1 Linguagem: Inglês

10.1186/s13059-018-1543-9

1474-760X

Sungsik Kim, Amos Chungwon Lee, Han‐Byoel Lee, Jinhyun Kim, Yushin Jung, Han Suk Ryu, Yong‐Ju Lee, Sang-Wook Bae, Minju Lee, Kyungmin Lee, Ryong Nam Kim, Woong‐Yang Park, Wonshik Han, Sunghoon Kwon,

Cell Image Analysis Techniques

Spatial mapping of genomic data to tissue context in a high-throughput and high-resolution manner has been challenging due to technical limitations. Here, we describe PHLI-seq, a novel approach that enables high-throughput isolation and genome-wide sequence analysis of single cells or small numbers of cells to construct genomic maps within cancer tissue in relation to the images or phenotypes of the cells. By applying PHLI-seq, we reveal the heterogeneity of breast cancer tissues at a high resolution and map the genomic landscape of the cells to their corresponding spatial locations and phenotypes in the 3D tumor mass.