Gene Discovery in Gelsemium Highlights Conserved Gene Clusters in Monoterpene Indole Alkaloid Biosynthesis
2018; Wiley; Volume: 20; Issue: 1 Linguagem: Inglês
10.1002/cbic.201800592
ISSN1439-7633
AutoresJakob Franke, Jeongwoon Kim, John P. Hamilton, Dongyan Zhao, Gina M. Pham, Krystle Wiegert‐Rininger, Emily Crisovan, Linsey Newton, Brieanne Vaillancourt, Evangelos C. Tatsis, C. Robin Buell, Sarah E. O’Connor,
Tópico(s)Phytochemical Studies and Bioactivities
ResumoAbstract Genome mining is a routine technique in microbes for discovering biosynthetic pathways. In plants, however, genomic information is not commonly used to identify novel biosynthesis genes. Here, we present the genome of the medicinal plant and oxindole monoterpene indole alkaloid (MIA) producer Gelsemium sempervirens (Gelsemiaceae). A gene cluster from Catharanthus roseus , which is utilized at least six enzymatic steps downstream from the last common intermediate shared between the two plant alkaloid types, is found in G. sempervirens , although the corresponding enzymes act on entirely different substrates. This study provides insights into the common genomic context of MIA pathways and is an important milestone in the further elucidation of the Gelsemium oxindole alkaloid pathway.
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