First Report of Banana Anthracnose Caused by Colletotrichum gloeosporioides in Ecuador
2018; American Phytopathological Society; Volume: 103; Issue: 4 Linguagem: Inglês
10.1094/pdis-01-18-0069-pdn
ISSN1943-7692
AutoresNadia Riera, Darío X. Ramírez-Villacís, Noelia Barriga-Medina, J. Alvarez-Santana, Karen L. Pulido-Herrera, Carlos Arturo David Ruáles, Antonio León-Reyes,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 103, No. 4First Report of Banana Anthracnose Caused by Colletotrichum gloeosporioides in Ecuador PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Banana Anthracnose Caused by Colletotrichum gloeosporioides in EcuadorN. Riera, D. Ramirez-Villacis, N. Barriga-Medina, J. Alvarez-Santana, K. Herrera, C. Ruales, and A. Leon-ReyesN. RieraLaboratorio de Biotecnología Agrícola y de Alimentos, Ingeniería en Agronomía, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Campus Cumbayá, Quito, Ecuador; , D. Ramirez-VillacisLaboratorio de Biotecnología Agrícola y de Alimentos, Ingeniería en Agronomía, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Campus Cumbayá, Quito, Ecuador; Instituto de Microbiología, Colegio de Ciencias Biológicas y Ambientales COCIBA Universidad San Francisco de Quito USFQ, Campus Cumbayá, Quito, Ecuador; , N. Barriga-MedinaLaboratorio de Biotecnología Agrícola y de Alimentos, Ingeniería en Agronomía, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Campus Cumbayá, Quito, Ecuador; Instituto de Microbiología, Colegio de Ciencias Biológicas y Ambientales COCIBA Universidad San Francisco de Quito USFQ, Campus Cumbayá, Quito, Ecuador; , J. Alvarez-SantanaLaboratorio de Biotecnología Agrícola y de Alimentos, Ingeniería en Agronomía, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Campus Cumbayá, Quito, Ecuador; , K. HerreraLaboratorio de Biotecnología Agrícola y de Alimentos, Ingeniería en Agronomía, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Campus Cumbayá, Quito, Ecuador; , C. RualesLaboratorio de Biotecnología Agrícola y de Alimentos, Ingeniería en Agronomía, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Campus Cumbayá, Quito, Ecuador; , and A. Leon-Reyes†Corresponding author: A. Leon-Reyes; E-mail: E-mail Address: [email protected]http://orcid.org/0000-0001-9142-9694Laboratorio de Biotecnología Agrícola y de Alimentos, Ingeniería en Agronomía, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Campus Cumbayá, Quito, Ecuador; Department of Biology, University of North Carolina at Chapel Hill, NC 27599-3280, U.S.A.AffiliationsAuthors and Affiliations N. Riera1 D. Ramirez-Villacis1 2 N. Barriga-Medina1 2 J. Alvarez-Santana1 K. Herrera1 C. Ruales1 A. Leon-Reyes1 4 † 1Laboratorio de Biotecnología Agrícola y de Alimentos, Ingeniería en Agronomía, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Campus Cumbayá, Quito, Ecuador; 2Instituto de Microbiología, Colegio de Ciencias Biológicas y Ambientales COCIBA Universidad San Francisco de Quito USFQ, Campus Cumbayá, Quito, Ecuador; 3Instituto de Investigaciones Biológicas y Ambientales BIÓSFERA, Colegio de Ciencias Biológicas y Ambientales COCIBA Universidad San Francisco de Quito USFQ, Campus Cumbayá, Quito, Ecuador; and 4Department of Biology, University of North Carolina at Chapel Hill, NC 27599-3280, U.S.A. Published Online:29 Jan 2019https://doi.org/10.1094/PDIS-01-18-0069-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In Ecuador, banana (Musa spp.) is the most important crop owing to its importance as a widely marketable fruit. In May 2014, in order to find pathogens observed during postharvest, banana fruit (Musa AAA Cavendish) collected from four organic plantations (under low input management) in the province of Los Ríos (Ecuador) were directly harvested (without postharvest treatment) and set in a humid chamber to evaluate the presence of disease symptoms. After 15 days banana fruit showed anthracnose-like symptoms. The symptoms observed began with small brown to reddish spots on the surface of the fruit and then became dark brown with sunken lesions. Fungal colonies with similar appearance were consistently isolated from lesions and grown on potato dextrose agar (PDA) medium at 27°C. All the isolates were subcultured applying the single-spore method. After 2 days, the isolates were grayish white at the beginning, and then they turned dark gray at the end of day 7. Under the microscope, we determined that conidia were hyaline and cylindrical to oblong with obtuse ends, with 15.8 ± 1.3 µm of length and 4.6 ± 0.5 µm of width. DNA from three independent isolates was used for further genetic analysis. The internal transcribed spacer (ITS), actin (ACT) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sequences were amplified (Weir et al. 2012). DNA sequences obtained from each marker were identical for the three isolates. Consensus sequences were built using ClustalX/MUSCLE under MEGA version 6 software (Tamura et al. 2013). BLAST search revealed 100% sequence homology with ITS (e-value, 0.0; accession KY342379), ACT (e-value, 2e-131; accession MH594288), and GAPDH (e-value, 3e-119; accession KT343752) sequences of Colletotrichum gloeosporioides. The consensus sequences were deposited in GenBank with the following accession numbers: ITS, MG564348; ACT, MH594288; and GAPDH, MH594287. A multilocus Bayesian inference phylogenetic tree (concatenated) was constructed using Beast version 1.8.4 to confirm the isolate identity (Drummond et al. 2012; Hyde et al. 2014; Weir et al. 2012). The isolate from banana clustered with isolates of C. gloeosporioides (stricto senso), which confirmed the identity of the isolate. Banana fruit were inoculated with C. gloeosporioides isolate. Fruit were surface sterilized with 70% ethanol followed by a wash of sterilized water. A wound of 1-mm diameter was made with a sterilized needle, and a drop of 5 µl of spore suspension (5.0 × 105 spores/ml) was placed on each wound independently. Six bananas per treatment were tested, and 5 µl of potato dextrose broth and sterile water were used as a control. All banana fruit were incubated in a humid chamber at 27°C under 12/12-h dark-light cycle. Similar symptoms were observed in all inoculated fruit after 3 days. Control banana fruit remained symptomless. The pathogen C. gloeosporioides was reisolated from the lesions, fulfilling Koch's postulates. The same pathogenicity assay was performed twice, showing similar results. Anthracnose in banana is known to be caused by C. musae, C. gloeosporioides, C. siamense, C. tropicale, C. chrysophilum, C. theobromicola, and C. scovillei (Kumar et al. 2017; Peres et al. 2001; Vieira et al. 2017; Zakaria et al. 2009; Zhou et al. 2017). To our knowledge, C. gloeosporioides has never been reported as pathogen causing anthracnose in banana fruit in Ecuador.References:Drummond, A. J., et al. 2012. Mol. Biol. Evol. 29:1969. https://doi.org/10.1093/molbev/mss075 Crossref, ISI, Google ScholarHyde, K. D., et al. 2014. Fungal Divers. 67:21. https://doi.org/10.1007/s13225-014-0298-1 Crossref, ISI, Google ScholarKumar, V. S., et al. 2017. Plant Dis. 101:390. https://doi.org/10.1094/PDIS-07-16-0961-PDN Link, ISI, Google ScholarPeres, N. A. R., et al. 2001. J. Phytopathology 150:128.https://doi.org/10.1046/j.1439-0434.2002.00732.x Crossref, ISI, Google ScholarTamura, K., et al. 2013. Mol. Biol. Evol. 30:2725. https://doi.org/10.1093/molbev/mst197 Crossref, ISI, Google ScholarVieira, W. A. S., et al. 2017. Mycologia 109:912. https://doi.org/10.1080/00275514.2017.1418577 Crossref, ISI, Google ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarZakaria, L., et al. 2009. Trop. Life Sci. Res. 20:119. Google ScholarZhou, Y., et al. 2017. Plant Dis. 101:381. https://doi.org/10.1094/PDIS-08-16-1135-PDN Link, ISI, Google ScholarGenetic Resource Access permit for this research was granted by the Ministry of Environment of Ecuador with the file number MAE-DNB-CM-2016-0046.N. Riera and D. Ramirez-Villacis contributed equally to this work.Funding: Funding was provided by Chancellor Grants USFQ and Poli Grants USFQ.DetailsFiguresLiterature CitedRelated Vol. 103, No. 4 April 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionGray mold on kiwifruit leaves caused by Botrytis cinerea (courtesy Guoshu Gong and Qinjun Tao); sunflower rust on bracts (courtesy Sam Markell); cucumber plant with mosaic symptoms caused by papaya ringspot virus (courtesy Roger Jones). Metrics Article History Issue Date: 10 Apr 2019Published: 29 Jan 2019First Look: 17 Oct 2018Accepted: 13 Oct 2018 Pages: 763-763 Information© 2019 The American Phytopathological SocietyFundingChancellor Grants USFQPoli Grants USFQCited byInhibitory effect and underlying mechanism of cinnamon and clove essential oils on Botryosphaeria dothidea and Colletotrichum gloeosporioides causing rots in postharvest bagging-free apple fruits27 February 2023 | Frontiers in Microbiology, Vol. 14Identification and Prevention of Microbial Contaminants in Musa paradisiaca Tissue Culture26 December 2022 | Malaysian Applied Biology, Vol. 51, No. 5Colletotrichum Species Complexes Associated with Crops in Northern South America: A Review23 February 2022 | Agronomy, Vol. 12, No. 3Identification of New Sources of Resistance to Anthracnose Caused by Colletotrichum horii among Persimmon Germplasms21 February 2022 | Horticulturae, Vol. 8, No. 2Colletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1Effectiveness of neutral electrolyzed water and copper oxychloride on fungi spores isolated from tropical fruitsHeliyon, Vol. 7, No. 9Multilocus phylogenetic analyses suggest the presence of Colletotrichum chrysophilum causing banana anthracnose in Mexico2 November 2020 | Journal of Plant Diseases and Protection, Vol. 128, No. 2Diversity of Colletotrichum Species Associated with Anthracnose Disease in Tropical Fruit Crops—A Review30 March 2021 | Agriculture, Vol. 11, No. 4Banana Pathology and Diseases24 July 2020Chemical composition and antifungal effect of ethanol extract from Sapindus saponaria L. fruit against banana anthracnoseScientia Horticulturae, Vol. 259
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