Produção Nacional
Revisado por pares
First Report of Groundnut Ringspot Orthotospovirus Infecting Soybeans in Brazil
2018; American Phytopathological Society; Volume: 103; Issue: 4 Linguagem: Inglês
10.1094/pdis-07-18-1246-pdn
ISSN1943-7692
AutoresM. G. Fontes, G. F. Andrews da Silva, M. F. Lima, M. E. N. Fonseca, Alexandre Florian da Costa, J. G. Silva-Filho, L. S. Boiteux,
Tópico(s)Plant Disease Management Techniques
ResumoHomePlant DiseaseVol. 103, No. 4First Report of Groundnut Ringspot Orthotospovirus Infecting Soybeans in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Groundnut Ringspot Orthotospovirus Infecting Soybeans in BrazilM. G. Fontes, G. F. Andrews da Silva, M. F. Lima, M. E. N. Fonseca, A. F. Costa, J. G. Silva-Filho, and L. S. BoiteuxM. G. FontesDepartamento de Fitopatologia, Universidade de Brasília, Brasília–DF, Brazil; , G. F. Andrews da SilvaUNESP, Botucatu–SP, Brazil; and , M. F. LimaEmbrapa Vegetable Crops (CNPH), Brasília–DF, Brazil, M. E. N. FonsecaEmbrapa Vegetable Crops (CNPH), Brasília–DF, Brazil, A. F. CostaEmbrapa Vegetable Crops (CNPH), Brasília–DF, Brazil, J. G. Silva-FilhoEmbrapa Vegetable Crops (CNPH), Brasília–DF, Brazil, and L. S. Boiteux†Corresponding author: L. S. Boiteux; E-mail: E-mail Address: leonardo.boiteux@embrapa.brhttp://orcid.org/0000-0002-5791-8002Embrapa Vegetable Crops (CNPH), Brasília–DF, BrazilAffiliationsAuthors and Affiliations M. G. Fontes1 G. F. Andrews da Silva2 M. F. Lima3 M. E. N. Fonseca3 A. F. Costa3 J. G. Silva-Filho3 L. S. Boiteux3 † 1Departamento de Fitopatologia, Universidade de Brasília, Brasília–DF, Brazil; 2UNESP, Botucatu–SP, Brazil; and 3Embrapa Vegetable Crops (CNPH), Brasília–DF, Brazil Published Online:4 Feb 2019https://doi.org/10.1094/PDIS-07-18-1246-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Soybean (Glycine max [L.] Merrill) is a major fabaceous crop in Brazil. In 2017, plants of soybean cultivar BRS 8180 RR were found exhibiting virus-like symptoms including apical mottling, mosaic, and chlorotic ringspots in three fields in Brasília–DF, Brazil. The overall disease incidence across the three fields was ≈5%. Seventy-two leaf samples (with and without virus-like symptoms) were collected in all three fields (24 samples per field). Samples were analyzed using nitrocellulose membrane enzyme linked immunosorbent assay with antisera (produced at CNPH) specific to the nucleocapsid (N) protein of three orthotospovirus species: tomato spotted wilt orthotospovirus (TSWV), tomato chlorotic spot orthotospovirus (TCSV), and groundnut ringspot orthotospovirus (GRSV). Twelve out of the 72 samples reacted positively only to GRSV-specific antiserum. To confirm the identity of the virus, total RNA was isolated from leaf samples of the 12 GRSV-positive plants using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA). Purified nucleic acids were used as template for the reverse transcription step. For cDNA synthesis, the J13 primer (5′-CCCGGATCCAGAGCAAT-3′), which encompasses eight conserved nucleotides at the 3′ termini of the three genomic RNAs (small [S], medium [M], and large [L]) of orthotospoviruses, was used. Polymerase chain reaction assays were then conducted with a set of virus-specific primers targeting the N gene of TSWV (5′-GCTGGAGCTAAGTATAGC-3′ and 5′-CACAAGGCAAAGACCTTGAG-3′) (Adkins and Rosskopf 2002), GRSV (5′-AGAGCTTCCTTAGTGTTGTACTTAG-3′ and 5′- GAAAGGTCTAGATCTAAACTGCCAC-3′) (Webster et al. 2011), and TCSV (5′- CTCGGTTTTCTGCTTTTC-3′ and 5′-CGGACAGGCTGGAGAAATCG-3′) (Baysal Gurel et al. 2015). Only GRSV-specific amplicons (≈600 bp) were obtained from all 12 GRSV-positive soybean plants. Amplicons of the three GRSV isolates S4 (MH388802), S8 (MH388803), and S30 (MG029625) were Sanger sequenced at CNPH. The consensus sequences were aligned and compared with other GRSV sequences from the database, using SDT version 1.2 (Muhire et al. 2014). Alignments of the N gene sequences of these GRSV isolates displayed nucleotide sequence identity above 95% with other GRSV isolates available at the GenBank database. In order to expand the genomic analysis of these isolates, we designed primers targeting the NSm gene (5′-TGACACTTTTCGGCAGCAA-3′ and 5′-CAAACACCTTCTTCTTCTTCC-3′) and the NSs gene (5′-ATAAGCACAAGAGCACAAG-3′ and 5′-CTGTAGCCATGAGCAAAGA-3′). The sequence alignments of the NSm (≈800 bp) (MH388796 to MH388798) and the NSs (≈702 bp) (MH388799 to MH388801) genes from the three soybean isolates displayed nucleotide identity above 95% with other GRSV isolates of a wide range of hosts. So far, only field peas and peanuts (Camelo-García et al. 2014) have been reported as fabaceous hosts of GRSV in Brazil. The persistent presence of viruliferous thrips (mainly Frankliniella schultzei [Trybom]; Thysanoptera: Thripidae) and alternative hosts belonging to native flora, solanaceous weeds, and cultivated plant species around/within soybean-planting areas in central Brazil might increase the importance of orthotospovirus to the crop. In addition, the identification of soybean as a new host of GRSV may impact crop rotation strategies, because this legume is often cultivated after processing tomatoes in this region.References:Adkins, S., and Rosskopf, E. N. 2002. Plant Dis. 86:1310. https://doi.org/10.1094/PDIS.2002.86.12.1310 Link, ISI, Google ScholarBaysal-Gurel, F., et al. 2015. Plant Dis. 99:163. https://doi.org/10.1094/PDIS-06-14-0639-PDN Link, ISI, Google ScholarCamelo-García, V. M., et al. 2014. J. Gen. Plant Pathol. 80:282. https://doi.org/10.1007/s10327-014-0518-2 Crossref, ISI, Google ScholarMuhire, B. M., et al. 2014. PLoS One 9:e108277. https://doi.org/10.1371/journal.pone.0108277 Crossref, ISI, Google ScholarWebster, C. G., et al. 2011. Virology 413:216. https://doi.org/10.1016/j.virol.2011.02.011 Crossref, ISI, Google ScholarFunding: Funding was provided by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Conselho Nacional de Desenvolvimento Científico e Tecnológico.DetailsFiguresLiterature CitedRelated Vol. 103, No. 4 April 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionGray mold on kiwifruit leaves caused by Botrytis cinerea (courtesy Guoshu Gong and Qinjun Tao); sunflower rust on bracts (courtesy Sam Markell); cucumber plant with mosaic symptoms caused by papaya ringspot virus (courtesy Roger Jones). Metrics Article History Issue Date: 10 Apr 2019Published: 4 Feb 2019First Look: 10 Oct 2018Accepted: 8 Oct 2018 Page: 777 Information© 2019 The American Phytopathological SocietyFundingCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorConselho Nacional de Desenvolvimento Científico e TecnológicoCited byMolecular and biological characterization of an isolate of the potyvirus passiflora virus Y naturally infecting soybean (Glycine max) in Brazil21 September 2022 | Archives of Virology, Vol. 167, No. 12Assessing the temporal dynamics of Frankliniella schultzei (Thysanoptera: Thripidae) in commercial soybean crops in North Brazil14 September 2021 | Agricultural and Forest Entomology, Vol. 24, No. 1Groundnut ringspot virusCABI Compendium, Vol. CABI CompendiumEvaluation of pyraclostrobin as a management tool of Groundnut ringspot virus in peanut crop6 August 2020 | Phytoparasitica, Vol. 48, No. 5First Report of Tomato Chlorotic Spot Virus in Soybean (Glycine max)C. Estévez de Jensen, J. E. Funderburk, T. Skarlinsky, and S. Adkins7 August 2019 | Plant Disease, Vol. 103, No. 10
Referência(s)