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Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression

2018; Taylor & Francis; Volume: 34; Issue: 7 Linguagem: Inglês

10.1080/08927014.2018.1504926

1029-2454

Ester Alves Ferreira Bordini, Caroline Coradi Tonon, Renata Serignoli Francisconi, Fernando Augusto Cintra Magalhães, Patricia Milagros Maquera-Huacho, Telma Blanca Lombardo Bedran, Sebastião Pratavieira, Luís Carlos Spolidório, Denise Madalena Palomari Spolidório,

Bacterial biofilms and quorum sensing

This study evaluated the antibacterial activity of terpinen-4-ol against Streptococcus mutans and Lactobacillus acidophilus and its influence on gbpA (S. mutans) and slpA (L. acidophilus) gene expression. As measured by XTT assay, the concentrations of terpinen-4-ol that effectively inhibited the biofilm were 0.24% and 0.95% for S. mutans and L. acidophilus, respectively. Confocal microscopy revealed the presence of a biofilm attached to the enamel and dentin block surfaces with significant terpinen-4-ol effects against these microorganisms. The expression of the gbpA and slpA genes involved in adherence and biofilm formation was investigated using RT-PCR. Expression of these genes decreased after 15 min with 0.24% and 0.95% terpinen-4-ol in S. mutans and L. acidophilus, respectively. These findings demonstrate the antimicrobial activity of terpinen-4-ol and its ability to modulate the expression of gbpA and slpA genes, emphasizing the therapeutic capacity of terpinen-4-ol as an alternative to inhibit adherence in biofilm.