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Reduced graphene oxide biosensor platform for the detection of NT-proBNP biomarker in its clinical range

2018; Elsevier BV; Volume: 126; Linguagem: Inglês

10.1016/j.bios.2018.09.102

1873-4235

Walid‐Madhat Munief, Xiaoling Lü, Tobias Teucke, Jannick Wilhelm, Anette Britz, Felix Hempel, Ruben Lanche, Miriam Schwartz, Jessica Ka Yan Law, Samuel Grandthyll, Frank Müller, Jens‐Uwe Neurohr, Karin Jacobs, Michael Schmitt, Vivek Pachauri, Rolf Hempelmann, Sven Ingebrandt,

Electrochemical sensors and biosensors

Reduced graphene oxide (rGO) thin films can be exploited as highly sensitive transducer layers and integrated in interdigital micro-electrode systems for biosensing processes. The distinctive bipolar characterisitics of rGO thin films can be modulated by a very low external electric field due to the electrostatic charges of biomolecules. These charges lead to a fast response in the readout signals of rGO based ion sensitive field-effect transistors (ISFETs). The characterisitc changes of rGO ISFETs enable a fast, accurate and reproducible detection of biomolecules. The biosensing mechanism offers a fast and label-free approach for analyte detection in contrast to the classical ELISA method. In this contribution, we introduce a reproducible fabrication process of rGO based field-effect transistors on wafer level. The sensors are functionalized as biosensors to measure N-terminal pro-brain natriuretic peptide (NT-proBNP) in human serum within its clinical range. Our optimized rGO sensor shows very promising electrical properties and can be considered as a proof of concept study for the detection of various analytes. The easy and cost-effective fabrication as well as the versatile usability make this new technological platform an auspicious tool for different sensing applications in future.