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DIVERSITY ASSESSMENT OF GRAPEFRUIT (CITRUS × PARADISI) AND TANGELO (CITRUS × TANGELO) UNDER INDIAN CONDITIONS USING PHYSICO-CHEMICAL PARAMETERS AND SSR MARKERS

2018; Hungarian University of Agriculture and Life Sciences; Volume: 16; Issue: 5 Linguagem: Inglês

10.15666/aeer/1605_53435358

1785-0037

S. M. Ahmed, H. S. Rattanpal, Gurteg Singh,

Plant biochemistry and biosynthesis

In a previous report, grapefruit (Citrus × paradisi Macfad.) and tangelo (Citrus × tangelo J.W. Ingram & H.E. Moore, 1975) landraces from diversity rich Indo-Gangetic Plain and arid regions of India were characterised using specific SSR markers.Eight grapefruit and two tangelo varieties were compared with germplasm of diverse origin comprising of cultivars, landraces and wild types.Significant differences were observed for different morphological characters studied.The highest phenotypic coefficient of variance (PCV) and genotypic co-efficient of variance (GCV) were determined for seed weight (104.4 and 103.7, respectively), number of seeds per fruit (102.3 and 101.9, respectively), number of fruits per tree (60.0 and 60.0, respectively) and acidity (40.4 and 38.8, respectively).These mentioned characters were more influenced by environment than other characters.A total of 75 alleles were amplified by 26 polymorphic simple sequence repeat (SSR) loci and the number of alleles ranged from 1 to 4 with an average of 2.88 alleles per locus.The highest number of alleles per locus was four as amplified by CAT01, CS05, CCSM204, CCSM70, CIBE5156, ATC09 and CMS26 followed by three alleles per locus each by CCSM77, CCSM156, CL11, OP29, CMS46, CMS09, CIBE4728, CMS3O and AG14 and the remaining markers amplified two alleles.The highest number of alleles among grapefruit varieties was found in Ray Ruby (75) while the lowest number of alleles was found in Flame (61) while among tangelo varities the highest number of alleles was found in Pearl (64).Average polymorphism (%) of all the polymorphic primer pairs across all the varieties of grapefruit was 69.55 and the highest polymorphism among grapefruit varieties was found in Marsh (82.09) while among tangelo varieties the highest polymorphism was found in Pearl (81.25).The measure of allelic diversity that is PIC value ranged from 0.17 (CS06) to 0.75 (CAT01) with an average value of 0.53.