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Activity and expression of E-NTPDase is altered in peripheral lymphocytes of systemic lupus erythematosus patients

2018; Elsevier BV; Volume: 488; Linguagem: Inglês

10.1016/j.cca.2018.10.032

1873-3492

Lara Vargas Becker, Renata da Silva Pereira Saccol, Vera Maria Morsch, Daniela Bitencourt Rosa Leal, Émerson André Casali, Nina Gabriela Müller Lopes, Valesca Veiga Cardoso, Maria Rosa Chitolina Schetinger,

Electrolyte and hormonal disorders

Systemic lupus erythematosus (SLE) is an inflammatory autoimmune disease, where there is irreversible breakdown of immunological self-tolerance. Extracellular adenosine triphosphate (ATP) and adenosine are signaling molecules that play an important part in the immune response. During inflammation and the immune response, a group of enzymes control these molecules, including ectonucleoside triphosphate diphosphohydrolase (E-NTPDase), E-5'-nucleotidase, and ecto-adenosine deaminase (E-ADA). We determined the activity and expression of E-NTPDase, the expression of E-5'-nucleotidase, the activity of E-ADA in lymphocytes and serum of SLE patients.This study involved 35 patients with SLE and 30 healthy subjects as a control group. E-NTPDase activity and expression were increased in lymphocytes from SLE patients (31% and 37% for activity and expression, respectively) compared with the control group.An approximately 42% increase in E-ADA activity in lymphocytes was observed in SLE patients compared with the control group, in serum the ADA activity was decreased by 57% in SLE patients. Expression of E-5'-nucleotidase was not changed in SLE patients.E-NTPDase and E-ADA perform key functions in the modulation of the immune and inflammatory response in SLE.