Produção Nacional
Revisado por pares
Capillary electrophoresis with dual diode array detection and tandem mass spectrometry to access cardiovascular biomarkers candidates in human urine: Trimethylamine-N-Oxide and l-carnitine
2018; Elsevier BV; Volume: 1583; Linguagem: Inglês
10.1016/j.chroma.2018.10.005
ISSN1873-3778
AutoresZuzana Cieslarová, Mariana Magaldi, Lucélia Alcantara Barros, Claudimir Lúcio do Lago, Daniel Rossado Oliveira, Francisco Antônio Helfenstein Fonseca, Maria Cristina de Oliveira Izar, Aline Soriano Lopes, Marina Franco Maggi Tavares, Aline Klassen,
Tópico(s)Microfluidic and Capillary Electrophoresis Applications
ResumoA capillary electrophoresis with diode array and tandem mass spectrometry detection (CE-UV-MS/MS) method has been developed for the targeted assessment of cardiovascular biomarkers candidates, trimethylamine-N-Oxide (TMAO) and l-carnitine, and creatinine in human urine samples. The dual detection was applied due to the high concentration of creatinine (monitored by UV detection at 200 nm) in relation to TMAO and l-carnitine (quantified by selected reaction monitoring (SRM) mass spectrometry), in human urine. All instrumental parameters, sheath liquid (SHL) and background electrolyte (BGE) compositions were optimized with a pool of urine provided by adult healthy volunteers and evaluated by signal-to-noise ratio (SNR) and peak shape of TMAO. The compositions for the optimized BGE was formic acid at concentration of 0.10 mol L-1, and for SHL was 70:30 MeOH:H2O containing 0.05% (v/v) formic acid, delivered at a flow rate of 5 μL min-1. Limits of detection for TMAO, l-carnitine and creatinine were 0.76, 0.54 and 303 μmol L-1, respectively. Limits of quantification were 2.5, 1.8 and 1000 μmol L-1, respectively. Linearity was evaluated by ANOVA and presented R2 from 0.993 to 0.997. Precision and accuracy were evaluated at three concentration levels. Coefficients of variation (CV) from 1 to 21% were obtained for the intra-day precision evaluation and from 2 to 16% for the inter-day precision evaluation. The recovery ranged from 75 to 116%. Quantitation of TMAO and l-carnitine in infarcted patients urine in comparison to healthy individuals indicated a 2.2 fold increase of TMAO and a 7.0 fold increase of l-carnitine. These results showed the potential applicability of the proposed method for the evaluation of TMAO and l-carnitine in urine within a panel of candidate metabolites in targeted metabolomics studies of cardiovascular diseases among other conditions.
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