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Metagenomic Sequencing of HIV-1 in the Blood and Female Genital Tract Reveals Little Quasispecies Diversity during Acute Infection

2018; American Society for Microbiology; Volume: 93; Issue: 2 Linguagem: Inglês

10.1128/jvi.00804-18

1098-5514

Anne Piantadosi, Catherine A. Freije, Christina Gosmann, Simon Ye, Daniel E. Park, S. F. Schaffner, Damien C. Tully, Todd M. Allen, Krista L. Dong, Pardis C. Sabeti, Douglas S. Kwon,

HIV/AIDS Research and Interventions

Due to error-prone replication, HIV-1 generates a diverse population of viruses within a chronically infected individual. When HIV-1 is transmitted to a new individual, one or a few viruses establish the new infection, leading to a genetic bottleneck in the virus population. Understanding the timing and nature of this bottleneck may provide insight into HIV-1 vaccine design and other preventative strategies. We examined the HIV-1 population in three women enrolled in a unique prospective cohort in South Africa who were followed closely during the earliest stages of HIV-1 infection. We found very little HIV-1 diversity in the blood and female genital tract during the first 2 weeks after virus was detected in the bloodstream. These results are compatible with a very early HIV-1 population bottleneck, suggesting the need to study the HIV-1 population in the female genital tract before virus is detectable in the bloodstream.