Portal de Periódicos da CAPES

The Trans Golgi Region is a Labile Intracellular Ca2+ Store Sensitive to Emetine

2018; Nature Portfolio; Volume: 8; Issue: 1 Linguagem: Inglês

10.1038/s41598-018-35280-z

2045-2322

Martín-Leonardo Gallegos-Gómez, Elisa Greotti, María-Cristina López-Méndez, Víctor-Hugo Sánchez-Vázquez, Juan Manuel Arias, Agustı́n Guerrero-Hernández,

Signaling Pathways in Disease

The Golgi apparatus (GA) is a bona fide Ca2+ store; however, there is a lack of GA-specific Ca2+ mobilizing agents. Here, we report that emetine specifically releases Ca2+ from GA in HeLa and HL-1 atrial myocytes. Additionally, it has become evident that the trans-Golgi is a labile Ca2+ store that requires a continuous source of Ca2+ from either the external milieu or from the ER, to enable it to produce a detectable transient increase in cytosolic Ca2+. Our data indicates that the emetine-sensitive Ca2+ mobilizing mechanism is different from the two classical Ca2+ release mechanisms, i.e. IP3 and ryanodine receptors. This newly discovered ability of emetine to release Ca2+ from the GA may explain why chronic consumption of ipecac syrup has muscle side effects.