First Report of Sugarcane Yellow Leaf Disease in Mexico and Detection of ‘ Candidatus Phytoplasma asteris’-Related Strains in Affected Plants
2018; American Phytopathological Society; Volume: 103; Issue: 5 Linguagem: Inglês
10.1094/pdis-09-18-1591-pdn
ISSN1943-7692
AutoresEdel Pérez‐López, Wei Wei, Robert E. Davis, J. Wang, Yan Zhao,
Tópico(s)Studies on Chitinases and Chitosanases
ResumoHomePlant DiseaseVol. 103, No. 5First Report of Sugarcane Yellow Leaf Disease in Mexico and Detection of ‘Candidatus Phytoplasma asteris’-Related Strains in Affected Plants PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Sugarcane Yellow Leaf Disease in Mexico and Detection of ‘Candidatus Phytoplasma asteris’-Related Strains in Affected PlantsE. Pérez-López, W. Wei, R. E. Davis, J. Wang, and Y. ZhaoE. Pérez-LópezDepartment of Biology, University of Saskatchewan, Saskatoon, SK, Canada; and Search for more papers by this author, W. WeiMolecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, U.S.A.Search for more papers by this author, R. E. Davishttp://orcid.org/0000-0003-4615-3101Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, U.S.A.Search for more papers by this author, J. WangMolecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, U.S.A.Search for more papers by this author, and Y. Zhao†Corresponding author: Y. Zhao; E-mail: E-mail Address: yan.zhao@ars.usda.govhttp://orcid.org/0000-0002-0032-7535Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, U.S.A.Search for more papers by this authorAffiliationsAuthors and Affiliations E. Pérez-López1 W. Wei2 R. E. Davis2 J. Wang2 Y. Zhao2 † 1Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada; and 2Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, U.S.A. Published Online:12 Mar 2019https://doi.org/10.1094/PDIS-09-18-1591-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Sugarcane is a common name for any of several tall perennial grass species of the genus Saccharum. As a major source for sugar production and an efficient feedstock for biofuel generation, sugarcane is widely cultivated in tropical and subtropical regions. Mexico alone produces over six million metric tons of cane sugar annually valued at 1.3 billion U.S. dollars. Although economically important, sugarcane is susceptible to devastating diseases caused by phloem-colonizing, cell wall-less bacteria known as phytoplasmas. Different sugarcane phytoplasmal diseases around the world have been attributed to diverse phytoplasmas belonging to six mutually distinct ‘Candidatus Phytoplasma’ species (Marcone 2002). During the 2015 to 2016 growing season, sugarcane plants exhibiting leaf discolorations (white and yellow streaks) indicative of sugarcane yellow leaf (ScYL) disease were observed in a sugarcane field in Cosamaloapan, Veracruz, Mexico, with less than 1% of the plants in the field being affected. Leaf samples were collected from three symptomatic and three asymptomatic plants in the same field. Total DNA was extracted from leaf midribs using a modified cetyltrimethylammonium bromide method (Pérez-López et al. 2016). A preliminary diagnostic assay was carried out using direct polymerase chain reactions (PCRs) with phytoplasma-specific primer pair R16F2n/R16R2. All PCR assays with DNA templates from symptomatic plants produced a phytoplasma-characteristic amplicon of 1.25 kb. No amplicon was detected from samples of asymptomatic plants. The DNA samples from symptomatic plants were subjected to further PCR analysis with primer pair P1A/16S-SR as previously described (Wei et al. 2011). All three samples were PCR positive, and each yielded a 1,539-bp amplicon. The amplicons were cloned and sequenced with at least 6× coverage per base position. DNA sequence analysis confirmed that the amplicon represented a near-full-length 16S rRNA gene and a partial 16S-23S RNA gene intergenic spacer. The obtained DNA sequences were deposited into GenBank (accession nos. MH891144 through MH891146). Analysis of the sequences through the iPhyClassifier (Zhao et al. 2009, https://plantpathology.ba.ars.usda.gov/cgi-bin/resource/iphyclassifier.cgi) revealed that the phytoplasmas detected in the ScYL-diseased plants were closely related to the reference strain of ‘Candidatus Phytoplasma asteris’ (aster yellows phytoplasma, >99.4% sequence similarity). Although the three 16S rRNA gene sequences differed from each other by two to five bases, all possessed the entire set of signature sequences (G196GGAGGA202, C444TGACGGTACC454, and C976ACAGTGGA GGTTATCAGTTG996) of ‘Ca. P. asteris’ (Lee et al. 2004), indicating the three ScYL phytoplasma strains are “sequevars” (Davis et al. 2015) affiliated with a single phytoplasma species. To our knowledge, this is the first report of phytoplasmal ScYL disease in Mexico. Associations of aster yellows phytoplasma with sugarcane diseases were previously reported in Cuba and Brazil (Arocha et al. 1999; Silva et al. 2009). Recently, infection of aster yellows phytoplasma in weedy grass growing near sugarcane fields in Mexico was noted (Pérez-López and Dumonceaux 2017). Findings of aster yellows phytoplasma infecting sugarcane in the countries of Brazil, Cuba, and now Mexico (this report) underscore the need for disease surveillance of sugarcane in neighboring countries, because insect vectors capable of spreading aster yellows phytoplasma strains are known to be present over wide areas, including the Caribbean countries and the United States.References:Arocha, Y., et al. 1999. Plant Dis. 83:1177. https://doi.org/10.1094/PDIS.1999.83.12.1177B Link, Google ScholarDavis, R. E., et al. 2015. Plant Dis. 99:1087. https://doi.org/10.1094/PDIS-11-14-1185-RE Link, ISI, Google ScholarLee, I.-M., et al. 2004. Int. J. Syst. Evol. Microbiol. 54:1037. https://doi.org/10.1099/ijs.0.02843-0 Crossref, ISI, Google ScholarMarcone, C. 2002. Sugar Tech 4:79. https://doi.org/10.1007/BF02942687 Crossref, Google ScholarPérez-López, E., and Dumonceaux, T. 2017. Trop. Plant Pathol. 43:242. https://doi.org/10.1007/s40858-017-0184-y Crossref, ISI, Google ScholarPérez-López, E., et al. 2016. Eur. J. Plant Pathol. 145:963. https://doi.org/10.1007/s10658-016-0883-0 Crossref, ISI, Google ScholarSilva, E. G., et al. 2009. J. Phytopathol. 157:771. https://doi.org/10.1111/j.1439-0434.2009.01561.x Crossref, ISI, Google ScholarWei, W., et al. 2011. Ann. Appl. Biol. 158:318. https://doi.org/10.1111/j.1744-7348.2011.00468.x Crossref, ISI, Google ScholarZhao, Y., et al. 2009. Int. J. Syst. Evol. Microbiol. 59:2582. https://doi.org/10.1099/ijs.0.010249-0 Crossref, ISI, Google ScholarE. Pérez-López and W. Wei contributed equally.DetailsFiguresLiterature CitedRelated Vol. 103, No. 5 May 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionTomato leaves infected with Tomato chlorotic dwarf viroid (Olmedo-Velarde, Roy, Belanger, Watanabe, Hamasaki, Mavrodieva, Nakhla, and Melzer). Photo credit: M. J. Melzer. Spinach plants with downy mildew symptoms caused by Peronospora effusa (Kandel, Mou, Shishkoff, Shi, Subbarao, and Klosterman). Photo credit: S. L. Kandel. Metrics Article History Issue Date: 8 May 2019Published: 12 Mar 2019First Look: 21 Nov 2018Accepted: 19 Nov 2018 Page: 1015 InformationThis article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2019.Cited byMolecular characterization of a 'Candidatus Phytoplasma asteris'-related strain (16SrI-B / cpn60UT I-IB) affecting daisies (Argyranthemum spp.) in Mexico6 February 2021 | Australasian Plant Disease Notes, Vol. 16, No. 1
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