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HPLC-PDA method validated for the determination of hibalactone in Hydrocotyle umbellata subterraneous parts and its ultrasound-assisted extraction optimization

2018; Springer Science+Business Media; Volume: 29; Issue: 2 Linguagem: Inglês

10.1016/j.bjp.2018.10.003

1981-528X

Matheus Gabriel de Oliveira, Paulo Henrique Gomes de Almeida, Thiago Levi Silva Oliveira, Liliane de Sousa Silva, Flávio Silva de Carvalho, Suzana Ferreira Alves, Leonardo Luiz Borges, Pierre Alexandre dos Santos, Vinícius Barreto da Silva, José Realino de Paula,

Alkaloids: synthesis and pharmacology

In this work, we developed and validated a HPLC-PDA method for the quantification of hibalactone in Hydrocotyle umbellata L., Araliaceae, subterraneous parts extracts and optimized its ultrasound-assisted extraction. Chromatographic separations were carried out with an isocratic mobile phase of acetonitrile/methanol/water (10:65:25), a flow of 0.8 ml min−1, detection at 290 nm and C18 column (250 × 4.6 mm, 5 μm). The method validation parameters were determined according to Brazilian legislation. The optimization of the hibalactone ultrasound-assisted extraction was performed using Box–Behnken design and response surface methodology. The HPLC method for hibalactone quantification proved to be selective, linear, precise, accurate and robust, being useful for the analysis of hibalactone in H. umbellata subterraneous parts extracts. The optimal ultrasound-assisted extraction conditions were obtained with solid-to-liquid ratio of 1:5 g ml−1, ethanolic strength of 70% (v/v) and temperature of 65 °C. The results can provide support of the quality control and standardization of raw materials from H. umbellata.