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DLK1 Is a Novel Link Between Reproduction and Metabolism

2018; Oxford University Press; Volume: 104; Issue: 6 Linguagem: Inglês

10.1210/jc.2018-02010

1945-7197

Larissa Gomes, Marina Cunha-Silva, Raiane P Crespo, Carolina Ramos, Luciana Ribeiro Montenegro, Ana Pinheiro Machado Canton, Melissa Lees, Helen Spoudeas, Andrew Dauber, Delanie B. Macedo, Danielle S. Bessa, Gustavo Arantes Rosa Maciel, Edmund Chada Baracat, Alexander A.L. Jorge, Berenice B. Mendonça, Vinícius Nahime Brito, Ana Cláudia Latronico,

Kruppel-like factors research

Delta-like homolog 1 (DLK1), also called preadipocyte factor 1, prevents adipocyte differentiation and has been considered a molecular gatekeeper of adipogenesis. A DLK1 complex genomic defect was identified in five women from a single family with central precocious puberty (CPP) and increased body fat percentage.We studied 60 female patients with a diagnosis of CPP or history of precocious menarche. Thirty-one of them reported a family history of precocious puberty. DLK1 DNA sequencing was performed in all patients. Serum DLK1 concentrations were measured using an ELISA assay in selected cases. Metabolic and reproductive profiles of adult women with CPP caused by DLK1 defects were compared with those of 20 women with idiopathic CPP.We identified three frameshift mutations of DLK1 (p.Gly199Alafs*11, p.Val271Cysfs*14, and p.Pro160Leufs*50) in five women from three families with CPP. Segregation analysis was consistent with the maternal imprinting of DLK1. Serum DLK1 concentrations were undetectable in three affected women. Metabolic abnormalities, such as overweight/obesity, early-onset glucose intolerance/type 2 diabetes mellitus, and hyperlipidemia, were more prevalent in women with the DLK1 mutation than in the idiopathic CPP group. Notably, the human metabolic alterations were similar to the previously described dlk1-null mice phenotype. Two sisters who carried the p.Gly199Alafs*11 mutation also exhibited polycystic ovary syndrome and infertility.Loss-of-function mutations of DLK1 are a definitive cause of familial CPP. The high prevalence of metabolic alterations in adult women who experienced CPP due to DLK1 defects suggests that this antiadipogenic factor represents a link between reproduction and metabolism.