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Improved reference genome of Aedes aegypti informs arbovirus vector control

2018; Nature Portfolio; Volume: 563; Issue: 7732 Linguagem: Inglês

10.1038/s41586-018-0692-z

1476-4687

Benjamin J. Matthews, Olga Dudchenko, Sarah B. Kingan, Sergey Koren, Igor Antoshechkin, Jacob E. Crawford, William J. Glassford, Margaret Herre, Seth Redmond, Noah H. Rose, Gareth D. Weedall, Yang Wu, Sanjit Singh Batra, Carlos A. Brito-Sierra, Steven D. Buckingham, Corey L. Campbell, Saki Chan, Eric Cox, Benjamin Evans, Thanyalak Fansiri, Igor Filipović, Albin Fontaine, Andrea Gloria‐Soria, Richard Hall, Vinita Joardar, Andrew K. Jones, Raissa G. G. Kay, Vamsi K. Kodali, Joyce Lee, Gareth Lycett, Sara N. Mitchell, Jill Muehling, Michael R. Murphy, Arina D. Omer, Frederick A. Partridge, Paul Peluso, Aviva Presser Aiden, Vidya Ramasamy, Gordana Rašić, Sourav Roy, Karla Saavedra-Rodríguez, Shruti Sharan, Atashi Sharma, Melissa Smith, Joe Turner, Allison M. Weakley, Zhilei Zhao, Omar S. Akbari, William C. Black, Han Cao, Alistair C. Darby, Catherine A. Hill, J. Spencer Johnston, Terence D. Murphy, Alexander S. Raikhel, David B. Sattelle, Igor V. Sharakhov, Bradley J. White, Li Zhao, Erez Lieberman Aiden, Richard S. Mann, Louis Lambrechts, Jeffrey R. Powell, Maria V. Sharakhova, Zhijian Tu, Hugh M. Robertson, Carolyn S. McBride, Alex Hastie, Jonas Korlach, Daniel E. Neafsey, Adam M. Phillippy, Leslie B. Vosshall,

CRISPR and Genetic Engineering

Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector.