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Molecular association of pathogenicity and resistance to multiple antimicrobials in Acinetobacter baumannii strains recovered from patients with diverse infectious diseases

2018; Sociedade Brasileira de Patologia Clínica; Volume: 54; Issue: 5 Linguagem: Inglês

10.5935/1676-2444.20180049

1678-4774

Rafaela Oliveira França, Priscila Simão Costa, Guilherme Luiz Milanez, Maria Rosa Quaresma Bomfim, Ricardo Gonçalves, Luiz M. Farias, Vandack Nobre, Simone Gonçalves dos Santos,

Identification and Quantification in Food

Introduction:The success of Acinetobacter baumannii infections can be attributed to its various virulence factors and antimicrobial resistance mechanisms.Objective: To evaluate the presence and correlation between different resistance and virulence factors in clinical A. baumannii strains.Methods: Study conducted at a University Hospital in Belo Horizonte, Minas Gerais, Brazil.The confirmation of Acinetobacter baumannii-calcoaceticus complex was performed by detecting the bla OXA-51 gene through the polymerase chain reaction (PCR), as well as the search for genes: bla OXA-23 , 24 , 58 , 143 , bla VIM-1 , csuE, ompA and ISAba1.Antimicrobials and metallo-betalactamase (MβL) expression were evaluated by E-test ® ; and genetic diversity, by enterobacterial repetitive intergenic consensus (ERIC)-PCR.Biofilm formation was classified into four categories according to the mean optical density obtained.Results: 98.4% (61/62) of the strains were resistant to meropenem; 71%, to ceftazidime; and 61.3%, to ampicillin-sulbactam; while 98.4% were sensitive to polymyxin B; and 48.4%, to tigecycline.The production of MβL was detected in 95.2% of the strains.The bla OXA-51 gene was detected in all strains tested; bla VIM-1 , in 83.9%; and ISAba1, in 90.3%.On the other hand, the csuE and ompA genes were present in 43.5% and 53.2% of the strains, respectively.Conclusion: There was a possible correlation between gentamicin resistant samples and those that were positive for the ompA gene.The csuE gene correlated positively with ISAba1.