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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage

2018; Multidisciplinary Digital Publishing Institute; Volume: 7; Issue: 12 Linguagem: Inglês

10.3390/jcm7120496

ISSN

2077-0383

Autores

Raimunda do Socorro da Silva Azevedo, Marialva Tereza Ferreira de Araújo, Consuelo Silva de Oliveira, Arnaldo Jorge Martins Filho, Bruno Nunes, Daniele Freitas Henriques, Eliana Silva, Valéria Lima Carvalho, Jannifer Oliveira Chiang, Livia Carício Martins, Barbara Cristina Baldez Vasconcelos, Jorge Rodrigues de Sousa, Fernanda Montenegro de Carvalho Araújo, Erlane Marques Ribeiro, A Rodriguez Castro, Maria De Queiroz, Mariana Pastorello Verotti, Márcio Roberto Teixeira Nunes, Ana Cecília Ribeiro Cruz, Suelí Rodrigues, Pei‐Yong Shi, Juarez Antônio Simões Quaresma, Robert B. Tesh, Pedro Fernando da Costa Vasconcelos,

Tópico(s)

Global Maternal and Child Health

Resumo

The recent Zika virus(ZIKV) epidemic in Brazil was characterized by a range of different clinical presentations, particularly microcephaly, Guillain-Barré syndrome, and death. In this context, we determined the causal relationship between fatal microcephaly cases and ZIKV infection.Twelve fatal cases of neonates, whose mothers were infected with ZIKV during pregnancy, were examined; cases included nine neonatal deaths due to microcephaly, one miscarriage, and two stillbirths. Tissue samples were obtained from all cases at necropsy and were submitted for virological investigation (RT-qPCR and virus isolation) and/or histopathology (hematoxylin and eosin staining) and immunohistochemical assay for the detection of ZIKV antigens.ZIKV antigens and/or ZIKV RNA were detected in tissue samples of all 12 cases examined. ZIKV was recovered in one case. Results of the virological and immunohistochemical analyses, as well as the anatomic abnormalities and histopathologic changes observed at necropsy on the 12 fatal cases, are presented.Data from these 12 cases provide strong evidence of the causal relationship between ZIKV and congenital disease in fetuses of women who were infected with the virus during pregnancy.

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