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Hydrophobin-stabilized nanoemulsion produced by a low-energy emulsification process: A promising carrier for nutraceuticals

2018; Elsevier BV; Volume: 89; Linguagem: Inglês

10.1016/j.foodhyd.2018.11.057

1873-7137

Christian Melo de Oliveira, Francisco Humberto Xavier Júnior, Andreza Rochelle do Vale Morais, Iasmim Lopes de Lima, Roberto Afonso Silva, André Ezequiel Gomes do Nascimento, Nathália Kelly de Araújo, Mariane Cajubá de Britto Lira Nogueira, Arnóbio Antônio da Silva-Júnior, Matheus de Freitas Fernandes‐Pedrosa, Eryvaldo Sócrates Tabosa do Egito,

Food Chemistry and Fat Analysis

Abstract Hydrophobin II (HFBII) is an amphiphilic biopolymer that could be explored to stabilize oil-in-water nanoemulsions as nutraceutical delivery systems. This study reports the production of HFBII-stabilized nanoemulsions by a spontaneous emulsification process using copaiba oil as a bioactive lipid. HFBII was isolated from a wild-type Trichoderma reesei and characterized. A 23 full factorial design with three central points was used to obtain an optimal nanoemulsion system, whose physical-chemical properties were studied under different ionic strength and pH. The peptide similarity search allowed the identification of a series of 6 ion fragments from the isolated fraction, which can be attributed to the amino acid sequences of the HFBII database. The optimal nanoemulsion system presented a nanoscale droplet size (