Produção Nacional
Revisado por pares
First Report of Blueberry Leaf Rust Caused by Thekopsora minima on Blueberry ( Vaccinium corymbosum ) in South America
2018; American Phytopathological Society; Volume: 103; Issue: 5 Linguagem: Inglês
10.1094/pdis-11-18-1981-pdn
ISSN1943-7692
AutoresPaulo César Pazdiora, Keilor da Rosa Dorneles, Jerônimo Vieira de Araújo Filho, Edemar Antonio Rossetto, Eduardo Guatimosim, Leandro José Dallagnol,
Tópico(s)Plant Pathogens and Fungal Diseases
ResumoHomePlant DiseaseVol. 103, No. 5First Report of Blueberry Leaf Rust Caused by Thekopsora minima on Blueberry (Vaccinium corymbosum) in South America PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Blueberry Leaf Rust Caused by Thekopsora minima on Blueberry (Vaccinium corymbosum) in South AmericaP. C. Pazdiora, K. R. Dorneles, J. V. Araújo Filho, E. A. Rossetto, E. Guatimosim, and L. J. DallagnolP. C. Pazdiora†Corresponding author: P. C. Pazdiora; E-mail Address: paulo.pazdiora@yahoo.com.brhttp://orcid.org/0000-0001-6996-9619Departamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil; and Search for more papers by this author, K. R. Dorneleshttp://orcid.org/0000-0001-8098-1539Departamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil; and Search for more papers by this author, J. V. Araújo FilhoDepartamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil; and Search for more papers by this author, E. A. RossettoDepartamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil; and Search for more papers by this author, E. GuatimosimInstituto de Ciências Biológicas, Universidade Federal do Rio Grande, São Lourenço do Sul, Rio Grande do Sul, BrazilSearch for more papers by this author, and L. J. DallagnolDepartamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil; and Search for more papers by this authorAffiliationsAuthors and Affiliations P. C. Pazdiora1 † K. R. Dorneles1 J. V. Araújo Filho1 E. A. Rossetto1 E. Guatimosim2 L. J. Dallagnol1 1Departamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil; and 2Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, São Lourenço do Sul, Rio Grande do Sul, Brazil Published Online:8 Mar 2019https://doi.org/10.1094/PDIS-11-18-1981-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Blueberry (Vaccinium corymbosum) is a plant native to North America, and it was introduced in Brazil in the 1980s in the State of Rio Grande do Sul. Leaves of blueberry showing leaf rust symptoms were observed on a commercial blueberry field, in the municipality of Jaguarão, Rio Grande do Sul, Brazil (32°39′80.93″S, 53°23′12.44″W) ranging from January to February 2017. The disease started as small reddish-brown leaf spots, epiphyllous, gregarious, with hypophyllous yellowish pustules. With disease progression, the leaf spots enlarged, becoming vein delimited, with a reddish-purple halo. At later stages, lesions coalesced, spreading all over the plant, causing severe precocious defoliation (especially on cultivars Jewel and Snowchaser). Pustules were produced abaxially, with abundant sporulation. Urediniospores were ovoid, yellowish-orange, echinulate, 15 to 24 × 10 to 18 μm (n = 53). Telia were not found. Genomic DNA was extracted from urediniospores and used for amplification of the internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS), using the primers ITS1-F_KYO2 and ITS4. The resulting fragment (465 bp) was sequenced and deposited in GenBank (MH029898). A BLAST search revealed 99% identity with Thekopsora minima (GenBank accession no. HQ661383). Aiming at verifying the correct diagnosis, a Bayesian phylogenetic inference was performed and showed that the Brazilian sample clustered with five other strains from different countries. To fulfill Koch’s postulates, suspension of urediniospores (1 × 103 spores/ml) was sprayed on leaves of healthy plants. Healthy plants sprayed with water served as a control. After inoculation, plants were kept inside a mist chamber at 95 to 100% relative humidity, temperature at 25°C, and 12-h photoperiod. Symptoms as small, reddish, irregular lesions appeared 7 days after inoculation, and pustules with uredospores and necrosis were observed at 21 days after inoculation. No symptoms occurred on control plants. To the best of our knowledge, this is the first report of blueberry leaf rust caused by T. minima on highbush blueberry in South America. The fungus has been reported on highbush blueberry in China, Japan, Mexico, South Africa, and the United States (Kobayashi 2007; Mostert et al. 2010; Rebollar-Alviter et al. 2011; Shands et al. 2018; Zheng et al. 2017). Although the yield losses were not measured in the present study, the severity observed at the field suggested that the intense and early defoliation significantly reduced the yield potential of the crop. Further studies are needed to properly evaluate the risk of blueberry leaf rust in commercial fields in Southern Brazil.References:Kobayashi, T. 2007. Page 1227 in: Index of Fungi Inhabiting Woody Plants in Japan: Host, Distribution and Literature. Zenkoku-Noson-Kyoiku Kyokai Publishing Co., Tokyo, Japan. Google ScholarMostert, L., et al. 2010. Plant Dis. 94:478. https://doi.org/10.1094/PDIS-94-4-0478C Link, ISI, Google ScholarRebollar-Alviter, A., et al. 2011. Plant Dis. 95:772. https://doi.org/10.1094/PDIS-12-10-0885 Link, ISI, Google ScholarShands, A. C., et al. 2018. Plant Dis. 102:1171. https://doi.org/10.1094/PDIS-09-17-1439-PDN Link, ISI, Google ScholarZheng, X., et al. 2017. Plant Dis. 101:835. https://doi.org/10.1094/PDIS-09-16-1379-PDN Link, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 103, No. 5 May 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionTomato leaves infected with Tomato chlorotic dwarf viroid (Olmedo-Velarde, Roy, Belanger, Watanabe, Hamasaki, Mavrodieva, Nakhla, and Melzer). Photo credit: M. J. Melzer. Spinach plants with downy mildew symptoms caused by Peronospora effusa (Kandel, Mou, Shishkoff, Shi, Subbarao, and Klosterman). Photo credit: S. L. Kandel. Metrics Article History Issue Date: 8 May 2019Published: 8 Mar 2019First Look: 4 Dec 2018Accepted: 27 Nov 2018 Pages: 1026-1026 Information© 2019 The American Phytopathological SocietyCited byPucciniastrum minimum is the causal agent of blueberry leaf rust on different Vaccinium species in Hawke’s Bay, New Zealand20 February 2023 | Australasian Plant Pathology, Vol. 47Pucciniastrum minimum (blueberry leaf rust)CABI Compendium, Vol. CABI CompendiumFirst report of Pucciniastrum minimum (syn. Thekopsora minima ) causing leaf rust on Vaccinium corymbosum (blueberry) in the United Kingdom and pathogenicity on Vaccinium myrtillus (bilberry)20 January 2022 | New Disease Reports, Vol. 45, No. 1First Report of Blueberry Leaf Rust Caused by Thekopsora minima on Vaccinium corymbosum in PeruM. Huarhua, R. Acuña, L. Aragón, J. Soto, S. Landeo, E. Martínez de la Parte, and W. Apaza31 August 2020 | Plant Disease, Vol. 104, No. 11Pest survey card on Thekopsora minimaEFSA Supporting Publications, Vol. 17, No. 8
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