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Induction of Porcine Dermatitis and Nephropathy Syndrome in Piglets by Infection with Porcine Circovirus Type 3

2018; American Society for Microbiology; Volume: 93; Issue: 4 Linguagem: Inglês

10.1128/jvi.02045-18

1098-5514

Haijun Jiang, Dan Wang, Jing Wang, Shanshan Zhu, Ruiping She, Xinxin Ren, Jijing Tian, Rong Quan, Lei Hou, Zixuan Li, Jun Chu, Yuxin Guo, Yanyang Xi, Huiqi Song, Yuan Feng, Wei Li, Jue Liu,

Virus-based gene therapy research

Porcine circovirus type 3 (PCV3), an emerging porcine circovirus, is considered the cause of porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs and other systemic diseases in piglets and sows. To evaluate the pathogenesis of PCV3 infection in vivo , we used a PCV3 virus stock from the rescue of an infectious PCV3 DNA clone to intranasally inoculate 4- and 8-week-old specific-pathogen-free piglets and demonstrated successful reproduction of PDNS-like disease in animals that were inoculated with PCV3 alone or PCV3 combined with immunostimulation by keyhole limpet hemocyanin. Both 4- and 8-week-old PCV3-inoculated piglets showed similar increases in viral loads in the sera and had seroconverted to PCV3 capsid antibody. Pathological lesions and PCV3-specific antigen were detected in various tissues and organs, while numerous proinflammatory cytokines and chemokines in the sera were significantly upregulated after PCV3 inoculation. These results will provide significant information regarding the pathogenesis of PCV3 in piglets.