Portal de Periódicos da CAPES

Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii)

2018; Elsevier BV; Volume: 502; Linguagem: Inglês

10.1016/j.aquaculture.2018.12.001

1873-5622

Carina Caldeira, Sandra Hernández-Ibáñez, Anthony Valverde, Patrick Martin, J.G. Herranz-Jusdado, Víctor Gallego, Juan F. Asturiano, Borys Dzyuba, Martin Pšenička, Carles Soler,

Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities

It is essential to define an optimized standard method to assess the fish sperm quality to minimize the differences between the results obtained by different laboratories. Only this optimization and standardization can make them useful from academia to industry. This study presents the validation of sperm motility assessment using a CASA-Mot system for three endangered diadromous fish species: European eel (Anguilla anguilla), Atlantic salmon (Salmo salar) and Siberian sturgeon (Acipenser baerii). To attain this goal, different technical and data processing methods were tested: 1) magnification lens (×10 and ×20), 2) Spermtrack® reusable chambers (10 and 20 μm depth) and 3) different frame rates (50 ≥ FR ≤ 250). The results suggested that the sperm motility assessment for eel, salmon and sturgeon should be performed at 200, 250 and 225 frames s−1, respectively. Moreover, to obtain a high number of analysed spermatozoa in less time and a natural movement of the sperm cells, it is recommended to use ×10 objective and 20 μm depth. In conclusion, different technical settings influence sperm kinetic parameters and should be validated for each fish species to allow the comparison of results between laboratories.