Produção Nacional
Revisado por pares
Comparative analysis of the antibacterial and drug-modulatory effect of d-limonene alone and complexed with β-cyclodextrin
2018; Elsevier BV; Volume: 128; Linguagem: Inglês
10.1016/j.ejps.2018.11.036
ISSN1879-0720
AutoresMaria do Socorro Costa, Janaína Esmeraldo Rocha, Fábia F. Campina, Ana Raquel Pereira da Silva, Rafael Pereira da Cruz, Raimundo Luíz Silva Pereira, Lucindo José Quintans‐Júnior, Irwin Rose Alencar de Menezes, Adriano Antunes de Souza Araújo, Thiago Sampaio de Freitas, Alexandre Magno Rodrigues Teixeira, Henrique Douglas Melo Coutinho,
Tópico(s)Enzyme Catalysis and Immobilization
ResumoWith the increase in bacterial resistance to antibiotics, many studies have been directed towards finding new agents with antibacterial activity, such as studies with natural products. These products can have antibacterial activity such as d-limonene as described in the literature. The aim of this study was to evaluate the antibacterial activity of d-limonene, isolated and complexed with β-cyclodextrin, and to evaluate its potentiating activity of different antibiotic classes. Antibacterial activity was determined by the broth microdilution method, obtaining in this way the Minimal Inhibitory Concentration (MIC), with the antibiotic modulatory activity being obtained using a sub-inhibitory concentration (MIC/8). d-Limonene showed a MIC equal to 256 μg/mL against standard S. aureus and 512 μg/mL against resistant P. aeruginosa. In the gentamicin modulatory activity, the isolated d-limonene presented synergism against S. aureus and E. coli bacteria. Thus, d-limonene showed relevant clinical antibacterial activity, for both Gram-positive and Gram-negative bacteria as well as a synergistic effect when associated with gentamicin. These results are promising in the combat against bacterial resistance, however further studies are needed to better elucidate the mechanisms of action.
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