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Description of Paraclostridium bifermentans subsp. muricolitidis subsp. nov., emended description of Paraclostridium bifermentans (Sasi Jyothsna et al., 2016), and creation of Paraclostridium bifermentans subsp. bifermentans subsp. nov

2018; Wiley; Volume: 63; Issue: 1 Linguagem: Inglês

10.1111/1348-0421.12663

1348-0421

Ryo Kutsuna, Tohru Miyoshi‐Akiyama, Koji Mori, Masahiro Hayashi, Junko Tomida, Yuji Morita, Kaori Tanaka, Yoshiaki Kawamura,

Bacterial Identification and Susceptibility Testing

Abstract Taxonomic studies of strain PAGU 1678 T , an obligately anaerobic, gram‐positive, spore‐forming bacterium isolated from biobreeding rat feces, were performed. This strain has been demonstrated to have the ability to exacerbate pathosis in a mouse model of dextran sulfate sodium‐induced ulcerative colitis. Phylogenetic analysis based on the 16S rRNA gene showed high homology with Paraclostridium bifermentans . To clarify the correct taxonomic position of strain PAGU 1678 T , a comparative taxonomic study using P. bifermentans PAGU 2008 T (═JCM 1386 T ) and the closely related bacterial species P. benzoelyticum PAGU 2068 T (═LMG 28745 T ) was carried out. Despite the close similarity of 16S rRNA gene sequences, DNA‐DNA hybridization between strain PAGU 1678 T and P. bifermentans PAGU 2008 T was 60.03% on average, average nucleotide identity was 96.17%, and it was shown to have different genomic sequences. Biochemically, strain PAGU 1678 T could be differentiated from P. bifermentans PAGU 2008 T by H 2 S production. Furthermore, strain PAGU 1678 T was characterized by the presence of two phospholipids with different polarity on polar lipid analysis. In addition, strain PAGU 1678 T differed from P. bifermentans PAGU 2008 T in findings on whole‐cell protein analysis and matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry. On the basis of these biochemical and genetic characteristics, a novel subspecies of P. bifermentans with the name Paraclostridium bifermentans subsp. muricolitidis subsp. nov. is here proposed, with PAGU 1678 T (═CCUG 72489 T ═NBRC 113386 T ) as the type strain, which automatically creates P. bifermentans subsp. bifermentans subsp. nov. JCM 1386 T (═ATCC 638 T ═DSM 14991 T ).