A red-emitting water-soluble fluorescent probe for biothiol detection with a large Stokes shift
2018; Elsevier BV; Volume: 30; Issue: 3 Linguagem: Inglês
10.1016/j.cclet.2018.12.017
ISSN1878-5964
AutoresLei Yang, Haiqing Xiong, Yuanan Su, Huihui Tian, Xingjiang Liu, Xiangzhi Song,
Tópico(s)Lanthanide and Transition Metal Complexes
ResumoIn this work, we presented a fluorescent probe (MCQ-DNBS) for selective and sensitive detection of biothiols based on a methylated chromenoquinoline (MCQ) derivative. Probe MCQ-DNBS was constructed by masking the OH group in MCQ with a common sensing unit, 2,4-dinitrobezensulfonate group (DNBS) for biothiols. Due to the photo-induced electron transfer (PET) process between MCQ and DNBS, this probe was weekly fluorescent. Upon the addition of biothiols (Cys, Hcy and GSH), this probe emitted a strong red fluorescence (λem max = 613 nm) with a large Stokes shift (115 nm). In addition, fluorescence imaging of biothiols in living cells was successfully realized using MCQ-DNBS as a detector.
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