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BIBTEX RIS

Evidence of selective pressure in whale fall microbiome proteins and its potential application to industry

2018; Elsevier BV; Volume: 45; Linguagem: Inglês

10.1016/j.margen.2018.11.004

1876-7478

Robert Cardoso de Freitas, Helena Isabel Ferreira Marques, Marcus Adonai Castro da Silva, Angélica Cavalett, Estácio Jussie Odisi, Bianca Linhares da Silva, Jessica Engel Montemor, Takashi Toyofuku, Chiaki Kato, Katsunori Fujikura, Hiroshi Kitazato, André Oliveira de Souza Lima,

Polar Research and Ecology

The present study addresses the microbiome of the first whale fall (YOKO 16) that has been described in the deep sea in the southern Atlantic Ocean (São Paulo Plateau; 4204 m depth), in terms of its metabolic uniqueness. Sets of ten thousand protein sequences from YOKO 16 and 29 public domain metagenomes (SRA and GenBank databases) that represent various marine, terrestrial and gut-associated microbial communities were analyzed. The determination of protein functionality, based on the KAAS server, indicated that the YOKO 16 microbiome has industrially-relevant proteins, such as proteases and lipases, that have low similarity (~50%) with previously-described enzymes. The amino acid usage in the YOKO 16 protein sequences (based on blastp and Clustal analysis) revealed a pattern of preference similar to that of extremophiles, with an increased usage of polar, charged and acidic amino acids and a decreased usage of nonpolar residues. We concluded that the targeted microbiome is of potential biotechnological use, which justifies the allocation of resources for the discovery of enzymes in deep-sea whale fall communities.