Ethanol represses the expression of methanol-inducible genes via acetyl-CoA synthesis in the yeast Komagataella phaffii
2018; Nature Portfolio; Volume: 8; Issue: 1 Linguagem: Inglês
10.1038/s41598-018-36732-2
ISSN2045-2322
AutoresShin Ohsawa, Susumu Nishida, Masahide Oku, Yasuyoshi Sakai, Hiroya Yurimoto,
Tópico(s)Fermentation and Sensory Analysis
ResumoAbstract In methylotrophic yeasts, the expression of methanol-inducible genes is repressed by ethanol even in the presence of methanol, a phenomenon called ethanol repression. The mechanism of ethanol repression in Komagataella phaffii ( Pichia pastoris ) was studied, and acetyl-CoA synthesis from ethanol by sequential reactions of alcohol dehydrogenase, aldehyde dehydrogenase and acetyl-CoA synthetase (ACS) was involved in ethanol repression. Molecular analysis of the ACS-encoding gene product KpAcs1 revealed that its N-terminal motif, which is conserved in methylotrophic yeasts, was required for ethanol repression. ACS activity was downregulated during methanol-induced gene expression, which partially depended on autophagy. In addition, acetyl-CoA synthesis and phosphorylation of a transcription factor KpMxr1 were found to contribute to ethanol repression in a synergistic manner.
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